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产品规格
检测灵敏度
0.03 pg/mL
测定范围
0.08-5.0 pg/ml
检测样本类型/体积
{Plasma=50 µL, Supernatant=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
6%
批内差异
4.9%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated microwell plate
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Control (High)
Control (Low)
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Control (High)
Control (Low)
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-6
均相（无需洗涤）
Human
检测试剂盒格式
High Sensitivity Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human High Sensitivity Interleukin 6 (HS IL-6) ELISA quantitates Hu HS IL-6 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-6.
原理方法
The Human HS IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
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生物信息学
基因别名 : BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6, IL6
基因ID : (Human) 3569
基因符号 : IL6
蛋白别名 : B-cell differentiation factor, B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6, Interferon beta-2, interleukin BSF-2, Interleukin-6
UniProt ID (Human) P05231
