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Human IgG Subclass ELISA Kit
To generate a 6-point standard curve, make serial dilutions of the standard using the Diluent Buffer. When reconstituted in 1 mL, the concentration of the standard is: 13.72 µg⁄mL of IgG1, 5.32 µg⁄mL of IgG2, 1.34 µg⁄mL of IgG3, and 0.76 µg⁄mL of IgG4. Below is the concentration of each IgG when diluted serially in half.
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产品规格
检测样本类型/体积
{Serum=10 µL}
手工操作时间
20 min
总时间
1 hr 10 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
2 x 96 Tests
产品成分
Pre-coated 96 well plates
Standard
Sample Diluent
Assay Buffer concentrate
Serum Control
MAbs to IgG Subclasses(4)
Peroxidase-conjugated Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Serum Control
MAbs to IgG Subclasses(4)
Peroxidase-conjugated Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Human IgG
Target sub-specificity
IgG1,IgG2,IgG3,IgG4
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human IgG Subclass Profile (Hu IgG) ELISA quantitates Hu IgG subclasses in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu igG1, IgG2, IgG3 or IgG4.
原理方法
The Human IgG ELISAs are designed to measure the amount of immunoglobulin in the sample. Each Immunoglobulin subunit, IgG1, IgG2, IgG3 and IgG4 are added to individual wells and adsorbed to the bottom of the microplate well. Samples, standards, or controls are then added into these wells and bind to the immobilized IgG subunit. Following a wash step, anti-human IgG antibodies conjugated to HRP is added. Following another wash step, a TMB substrate solution is added that reacts with the complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Human IgG, also known as human immunoglobulin G, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in the blood and plays a key role in defense against pathogens. IgG works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
