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产品规格
检测灵敏度
2.31 pg/mL
测定范围
15.6-1,000 pg/mL
检测样本类型/体积
{Amniotic Fluid=20 µL, Plasma=20 µL, Serum=20 µL, Supernatant=20 µL}
手工操作时间
15 min
总时间
3 hr 10 min
均相（无需洗涤）
No
批间差异
6.2%
批内差异
7.7%
检测仪器
Colorimetric Microplate Reader
产品规格
128 Tests
产品成分
Pre-coated 96 well Microplate
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
运输条件
Dry ice
储存
-20°C
蛋白名称
CCL2 (MCP-1)
均相（无需洗涤）
Human
检测试剂盒格式
Instant ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Monocyte Chemoattractant Protein 1 (MCP-1) ELISA quantitates Hu MCP-1 in human serum, plasma, cell culture supernatants,amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu MCP-1.
原理方法
The Human MCP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF
基因ID : (Human) 6347
基因符号 : CCL2
蛋白别名 : C-C motif chemokine 2, chemokine (C-C motif) ligand 2, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, Small-inducible cytokine A2
UniProt ID (Human) P13500
