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The Human MIP-3 alpha/CCL20 ELISA quantitates Hu MIP-3α in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIP-3α.
原理方法
The Human MIP-3α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : CCL20, CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38
基因ID : (Human) 6364
基因符号 : CCL20
蛋白别名 : beta chemokine exodus-1, Beta-chemokine exodus-1, C-C motif chemokine 20, CC chemokine LARC, chemokine (C-C motif) ligand 20, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, small inducible cytokine subfamily A (Cys-Cys), member 20, Small-inducible cytokine A20
UniProt ID (Human) P78556