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产品规格
检测灵敏度
4 pg/mL
测定范围
10-1000 pg/mL
检测样本类型/体积
{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL, Tissue Homogenate=10 µL, Urine=10 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 10 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer Concentrate
Chromogen
Stop Solution
Controls
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer Concentrate
Chromogen
Stop Solution
Controls
运输条件
Wet ice
储存
2-8°C
蛋白名称
NGAL
蛋白家族
Growth Factors & Receptors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human NGAL (Hu Neutrophil Gelatinase-Associated Lipocalin) ELISA quantitates Hu NGAL in human serum, plasma, urine, tissue homogenates, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NGAL.
原理方法
The Human NGAL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : 24p3, HNL, LCN2, MSFI, NGAL, p25
基因ID : (Human) 3934
基因符号 : LCN2
蛋白别名 : 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, migration-stimulating factor inhibitor, Neutrophil gelatinase-associated lipocalin, NGAL, Oncogene 24p3, p25, Siderocalin, siderocalin LCN2
UniProt ID (Human) P80188
