Search Thermo Fisher Scientific
The Human Osteocalcin ELISA quantitates Hu Osteocalcin in human serum and plasma,. The assay will exclusively recognize both natural and recombinant Hu Osteocalcin.
原理方法
The Human Osteocalcin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : BGLAP, BGP, OC, OCN
基因ID : (Human) 632
基因符号 : BGLAP
蛋白别名 : BGP, bone gamma-carboxyglutamate (gla) protein (osteocalcin), Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, Osteocalcin
UniProt ID (Human) P02818