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![Human PRAS40 (Phospho) [pT246] ELISA Kit](https://www.thermofisher.cn/antibody/images/650/4102.jpg?time=20220826 "Human PRAS40 (Phospho) [pT246] ELISA Kit")
![Human PRAS40 (Phospho) [pT246] ELISA Kit](https://www.thermofisher.cn/antibody/images/150/4102.jpg?time=20220826)
![Human PRAS40 (Phospho) [pT246] ELISA Kit](https://www.thermofisher.cn/antibody/images/150/KHO0421_SC_080324.jpg?time=20220826)
![Human PRAS40 (Phospho) [pT246] ELISA Kit (KHO0421)](https://www.thermofisher.cn/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20220826)
产品规格
检测灵敏度
<0.5 U/mL
测定范围
1.6-100 U/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
5.9%
批内差异
4.7%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
PRAS40
Target site-specificity
Phosphorylated Thr246
蛋白亚型
Adaptor Proteins
蛋白家族
Kinases & Inhibitors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human PRAS40 (Phospho) [pT246] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of PRAS40 (Phospho) [pT246] in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant human PRAS40 (Phospho) [pT246].
原理方法
A monoclonal capture antibody specific for PRAS40 (Phospho) [pT246] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Treatment with the insulin-like growth factor-1 (IGF1) can induce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : AKT1S1, Lobe, PRAS40
基因ID : (Human) 84335
基因符号 : AKT1S1
蛋白别名 : 40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline rich), AKT1 substrate 1 (proline-rich), AKT1S1, Proline-rich AKT1 substrate 1
UniProt ID (Human) Q96B36
