Search Thermo Fisher Scientific
The Renin Human ELISA Kit quantitates Hu Renin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Renin.
原理方法
The Human Renin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : HNFJ2, REN
基因ID : (Human) 5972
基因符号 : REN
蛋白别名 : angiotensin-forming enzyme, Angiotensinogenase, Kidney renin, D19352, Ren, Ren-1, Ren-Ac, Ren1d, Rn-1, Rnr, Ren1, Renin, renin precursor, renal
UniProt ID (Human) P00797