技术指南和实验方案 技术支持

产品规格

检测灵敏度

<2 pg/mL

测定范围

15.6-1000 pg/mL

检测样本类型/体积

{Cerebrospinal Fluid=50 µL, Supernatant=50 µL}

手工操作时间

1 hr 20 min

总时间

5 hr

均相（无需洗涤）

No

批间差异

6.2%

批内差异

4.2%

检测仪器

Colorimetric Microplate Reader

产品规格

96 Tests

产品成分

Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

运输条件

Wet ice

储存

2-8°C

蛋白名称

Tau

Target site-specificity

Phosphorylated Ser396

均相（无需洗涤）

Human

检测试剂盒格式

Sandwich ELISA Kit

检测抗体结合物

HRP

标签或染料

HRP

关于本试剂盒

The Human Tau (Phospho) [pS396] ELISA quantitates Hu Tau (Phospho) [pS396] in human cerebrospinal fluid (CSF), buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Tau (Phospho) [pS396].

原理方法

The Human Tau (Phospho) [pS396] solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

严格验证

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

靶标信息

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

基因别名 : DDPAC, FTDP-17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU

基因ID : (Human) 4137

基因符号 : MAPT

蛋白别名 : G protein beta1/gamma2 subunit-interacting factor 1, Microtubule-associated protein tau, microtubule-associated protein tau, isoform 4, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, protein phosphatase 1, regulatory subunit 103

UniProt ID (Human) P10636

guarantee_icon

性能保障

只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符，您即可享受免费退换货服务。*

了解更多
help_icon

随时为您服务

获取常见问题的专家解答，或者直接联系我们的技术专家，咨询应用、设备、常规产品使用问题。

联系技术支持