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产品规格
检测样本类型/体积
{Supernatant=10 µL}
手工操作时间
30 min
总时间
2 hr 15 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
2 x 96 Tests
产品成分
Pre-coated 96 well Microplate coated with Streptavidin
Biotinylated monoclonal anti-mouse isotype or light chain-specific capture antibodies (Isotype-specific: anti-IgG1, IgG2a, IgG2b, IgG2c, IgA, IgM; Light chain specific: lambda or kappa)
HRP-Conjugated Anti-Rat lg (H&L) polyclonal antibody
Sample Diluent, lyophilized
Mouse Positive Control: Mouse IgG1, IgG2a, IgG2b, IgG2c, IgA, IgM, kappa and lambda isotype control mix (lyophilized)
Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers
Biotinylated monoclonal anti-mouse isotype or light chain-specific capture antibodies (Isotype-specific: anti-IgG1, IgG2a, IgG2b, IgG2c, IgA, IgM; Light chain specific: lambda or kappa)
HRP-Conjugated Anti-Rat lg (H&L) polyclonal antibody
Sample Diluent, lyophilized
Mouse Positive Control: Mouse IgG1, IgG2a, IgG2b, IgG2c, IgA, IgM, kappa and lambda isotype control mix (lyophilized)
Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
Mouse Ig
Target sub-specificity
Ig kappa light chain,Ig lambda light chain,IgA,IgG1,IgG2a,IgG2b,IgG3,IgM
均相（无需洗涤）
Mouse
检测试剂盒格式
Instant ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse Immunoglobulin Isotyping ELISA qualitatively detects Mouse Ig isotypes in mouse hybridoma cell culture supernatants.
原理方法
Biotinylated monoclonal anti-mouse isotype specific or light chain specific capture antibodies bind to the Streptavidin coated plate. Ig Isotype present in the positive control or sample binds to the specific capture antibodies and is simultaneously bound by the HRP-conjugated detector antibody. Following incubation unbound reagents and sample components are removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of soluble mouse Ig (H+L) present in the sample.
严格验证
Each manufactured lot of this ELISA kit is quality tested. See manual for more information on validation.
靶标信息
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
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