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产品规格
检测灵敏度
4.98 ng/mL
测定范围
12.5-800 ng/mL
检测样本类型/体积
{Urine=50 µL, Saliva=50 µL, Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Corticosterone
Species (reported)
Mouse
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Mouse Corticosterone (CORT) ELISA quantitates CORT in serum, plasma, urine, saliva.
原理方法
The Mouse Cort solid phase competitive ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between the pre coated antigen and the HRP linked antibody specific for Mouse Cort. A target specific antigen has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells, where Mouse Cort in the samples (or standards) competes with the immobilized (capture) antigen for sites on the HRP linked detection antibody. Excess conjugate and unbound sample or standard are washed away from the plate. The substrate solution is added to each well, and the enzyme substrate reaction is terminated by the addition of a stop solution, turning the color yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The intensity of this signal is inversely proportional to the concentration of Mouse Cort present in the original specimen, which is determined by comparing the OD of the samples to the standard curve.
Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
CORTICOSTERONE is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid. [National Center for Biotechnology Information. PubChem Compound Database; CID=5753].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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