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The Mouse Cystatin C (CST3) ELISA quantitates Ms Cystatin C in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Cystatin C.
原理方法
The Mouse Cystatin C solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Cystatin C, a member of the type 2 cystatin superfamily, is a potent inhibitor of cysteine proteinases, both endogenous (lysosomal cathepsins), and pathogenic. Cystatin C is a 13 kDa secreted protein which is found in high concentrations in biological fluids and is thought to be expressed by all human nucleated cells. Changes in Cystatin C have been documented in a number of cardiovascular diseases, including atherosclerosis, amyloid angiopathy and myocardial infarction. Its low molecular weight also means that Cystatin C can be used as a marker of renal failure.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : Cst3, CysC
基因ID : (Mouse) 13010
基因符号 : Cst3
蛋白别名 : cystatin 3, Cystatin-3, Cystatin-C
UniProt ID (Mouse) P21460