Search Thermo Fisher Scientific
产品规格
检测灵敏度
9.38 pg/mL
测定范围
15.63-1000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
BNP
蛋白家族
Natriuretic peptide
均相（无需洗涤）
Mouse
检测试剂盒格式
Competitive ELISA
标签或染料
HRP
关于本试剂盒
The Mouse Brain Natriuretic Peptide (BNP) ELISA quantitates BNP in serum, plasma and other biological fluids
原理方法:
The Competitive Brain Natriuretic Peptide (BNP) ELISA research-use-only kit is designed to quantitatively measure BNP in mice. A BNP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Mouse BNP is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Mouse BNP in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
基因别名 : AA408272, BNF, BNP, Nppb
基因ID : (Mouse) 18158
基因符号 : Nppb
蛋白别名 : Brain natriuretic factor prohormone, brain natriuretic peptide, Gamma-brain natriuretic peptide, Iso-ANP, natriuretic peptide precursor type B, Natriuretic peptides B, preproBNP, type-B natriuretic factor
UniProt ID (Mouse) P40753
