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The Mouse P-Selectin ELISA quantitates Ms P-Selectin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms P-Selectin.
原理方法
The Mouse P-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : CD62P, GMP-140, Grmp, LECAM3, PADGEM, Selp
基因ID : (Mouse) 20344
基因符号 : Selp
蛋白别名 : CD62 antigen-like family member P, CD62P, GMP-140, Granule membrane protein 140, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, P-selectin, PADGEM, Platelet activation dependent granule-external membrane protein
UniProt ID (Mouse) Q01102