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产品规格
检测灵敏度
0.75 pg/mL
测定范围
3.13-200 pg/ml
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL, Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
9.2%
批内差异
4.7%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated microwell plate
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Calibrator Diluent
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Calibrator Diluent
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
TNF alpha
均相（无需洗涤）
Mouse
检测试剂盒格式
High Sensitivity Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse High Sensitivity Tumor Necrosis Factor alpha (HS TNFα) ELISA quantitates Ms HS TNFα in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms HS TNFα.
原理方法
The Mouse HS TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : DIF, Tnf, TNF-a, TNF-alpha, Tnfa, TNFalpha, Tnfsf1a, TNFSF2
基因ID : (Mouse) 21926
基因符号 : Tnf
蛋白别名 : Cachectin, TNF-a, TNF-alpha, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
UniProt ID (Mouse) P06804
