Search Thermo Fisher Scientific
产品规格
检测灵敏度
1.98 pg/mL
测定范围
0.16-10.0 ng/mL
检测样本类型/体积
{Cerebrospinal Fluid=10 µL, Lavage Fluid=10 µL, Plasma=10 µL, Supernatant=10 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 20 min
均相（无需洗涤）
No
批间差异
5.6%
批内差异
4.8%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Neutrophil elastase
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human PMN (Neutrophil) Elastase) ELISA quantitates Hu PMN (Neutrophil) Elastase in human plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant PMN (Neutrophil) Elastase.
原理方法
The Human PMN (Neutrophil) Elastase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : ELA2, ELANE, GE, HLE, HNE, NE, PMN-E, SCN1
基因ID : (Human) 1991
基因符号 : ELANE
蛋白别名 : Bone marrow serine protease, elastase 2, neutrophil, Elastase-2, granulocyte-derived elastase, HLE, Human leukocyte elastase, leukocyte elastase, Medullasin, Neutrophil elastase, PMN elastase, polymorphonuclear elastase
UniProt ID (Human) P08246
