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产品规格
测定范围
31.3-4000 pg/mL
检测样本类型/体积
{Plasma=100 µL, Urine=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
11.7%
批内差异
6%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Tris Buffer
Alkaline Phosphatase Tracer
Leukotriene B4 Antiserum
DEA Buffer
Wash Buffer
pNPP tablets
Adhesive Plate Covers
Standard
Tris Buffer
Alkaline Phosphatase Tracer
Leukotriene B4 Antiserum
DEA Buffer
Wash Buffer
pNPP tablets
Adhesive Plate Covers
运输条件
Dry ice
储存
Store kit at -20°C. Refer to product documentation for component specific storage temperature.
蛋白名称
PGE2
均相（无需洗涤）
Human
检测试剂盒格式
Competitive ELISA
标签或染料
AP
关于本试剂盒
The Human Prostaglandin E2 ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of human prostaglandin E2 in biological samples.
原理方法
This assay is based on the competition between Prostaglandin E 2 (PGE2) and a PGE2 alkaline phosphatase tracer for a limited amount of PGE2 specific monoclonal antibody. Because of the competition between PGE2 in the sample and PGE2 tracer for the PGE2 antibody, the signal obtained with the assay will be inversely proportional to the amount of PGE 2 in each sample.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Prostaglandin E2 (PGE2) is a lipid mediator derived from arachidonic acid through the actions of cyclooxygenase (COX) enzymes and terminal prostaglandin E synthases (PGES). It plays a significant role in mediating inflammation, pain sensation, and fever by acting on four receptor subtypes: EP1, EP2, EP3, and EP4. PGE2's involvement in inflammatory responses is well-documented, particularly in diseases such as rheumatoid arthritis and osteoarthritis. Various medications, including nonsteroidal anti-inflammatory drugs (NSAIDs) and selective COX-2 inhibitors, are used to reduce PGE2 production, thereby alleviating inflammation and pain. However, these drugs can have side effects, such as gastrointestinal issues and increased risk of thrombosis. Additionally, PGE2 has a critical role in immune regulation, affecting T cell function and, by extension, the adaptive immune response. It also has a significant impact on skeletal disorders and pain through mechanisms that are still being explored.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名 : C20H32O5, PGE2
