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The Human Prostaglandin E2 ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of human prostaglandin E2 in biological samples.
原理方法
This assay is based on the competition between Prostaglandin E 2 (PGE2) and a PGE2 alkaline phosphatase tracer for a limited amount of PGE2 specific monoclonal antibody. Because of the competition between PGE2 in the sample and PGE2 tracer for the PGE2 antibody, the signal obtained with the assay will be inversely proportional to the amount of PGE 2 in each sample.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Prostoglandin E2 (PGE2) is a potent lipid mediator produced by metabolism of arachidonic acid via the cyclooxygenase (COX) pathway. Following cellular stimulation, arachidonic acid is hydrolyzed from phospholipids stores by one of a family of phospholipase A2 enzymes and converted to the unstable endoperoxide PGH2 by either COX 1 or COX 2. PGH2 is isomerized to PGE2 by one of 3 distinct enzymes microsomal PGES 1 (mPGES 1), mPGES 2, or a cytosolic enzyme, cPGES. PGE2 is active in a number of physiological systems and settings including inflammation, immune regulation, generation of fever, pain perception, protection of the gastric muscosa, fertility and parturition, as well as sodium and water retention. The effects of PGE2 are transduced by 4 subtypes of G protein coupled receptors designated EP1, EP2, EP3, EP4, with Kd values for PGE2 ranging from 1 10 nM. PGE2 is rapidly metabolized in vivo by the prostaglandin 15 dehydrogenase pathway (15 hydroxy PGDH) to the inactive metabolite 13,14 dihydro 15 keto PGE2. The half life of PGE2 in the circulatory system is ~30 seconds and normal plasma levels are 3 12 pg/mL
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : C9orf15, GBF-1, GBF1, mPGES-2, PGES2, PTGES2
基因ID : (Human) 80142
基因符号 : PTGES2
蛋白别名 : C20H32O5, PGE2, gamma-interferon-activated transcriptional element-binding factor 1, GATE-binding factor 1, membrane-associated prostaglandin E synthase 2, Membrane-associated prostaglandin E synthase-2, Microsomal prostaglandin E synthase 2, microsomal prostaglandin E synthase-2, mPGE synthase-2, mPGES-2, Prostaglandin E synthase 2, Prostaglandin-H(2) E-isomerase
UniProt ID (Human) Q9H7Z7