LinkSeq PCR Typing Kits

Fast and easy real-time PCR genotyping


Thermo Scientific LinkSēq is a rapid genotyping method with a simple workflow that prevents post-amplification contamination. With a fast turnaround time in as little as 90 minutes, and minimal hands-on time, your lab’s productivity will greatly increase.


High throughput can be achieved by using a thermal cycler for the first 60 minutes of the amplification step and only 10 minutes on a real-time PCR instrument, so multiple samples can be typed within that hour. Our product line includes HLA genotyping of HLA and KIR, and blood genotyping of RH, ABO, and HPA.

 

LinkSēq workflow – as little as 90 minutes to results

LinkSēq HLA Typing Kits

Our LinkSēq reagents provide the most complete solution for high throughput laboratories interested in fast and easy typing of all 11 loci. This product provides low resolution typing for HLA A, B, C, DRB1, DRB345, DQA1, DQB1, DPA1, and DPB1, as well as common null alleles.

Features

  • Molecular detection features SYBR Green Real-Time PCR
  • All necessary reagents are included in the kit
  • No contamination control needed
  • As little as 10 minutes of hands-on
  • Genotyping results of Class I and II loci in as little as 90 minutes
  • Fewer repeats, faster workflow steps, and reproducible results

LinkSēq HLA - specific genetic markers

These kits type specific HLA alleles such as B27 or B*57:01. Each kit types a few specific alleles in strip format. This is a fast, efficient an cost effective solution for labs looking for specific typing of the alleles included in the strip.
 

LinkSēq KIR Typing Kits

Killer cell immunoglobulin-like receptors (KIR) are a family of cell surface receptors found on human Natural Killer (NK) cells. Certain KIR types interact with HLA Class I molecules to inhibit the activity of NK cells while other KIR types can stimulate NK activity. The Thermo Scientific LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others.

 

Features

  • Available in 96- and 384-well trays.
  • All necessary reagents included in kit
  • No contamination control needed
  • As little as 10 minutes of hands-on
  • Genotyping results of Class I and II loci in as little as 90 minutes
  • Fewer repeats, faster workflow steps, and reproducible results

LinkSēq ancillary

Our accessories support the use of the core LinkSēq products and a key ancillary product is the Thermo Scientific LinkSēq Wipe Test. According to widely accepted Standards and Guidelines (e.g. ASHI and EFI), laboratories must routinely monitor for contamination of pre-amplification areas by the most common amplification products that are produced in the laboratory.
 

The LinkSēq Wipe Test was developed to detect both genomic DNA contamination as well as potential LinkSēq amplicon contamination. Additional products include accessories to ensure that your needs are covered.

 

Not all products are CE marked or have 510(k) clearance for sale in the U.S. Availability of products in each country depends on local regulatory marketing authorization status.