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DNA melting temperature analysis
Double-stranded DNA can play an important role in a variety of pharmaceutical formulation procedures, including vaccine development. The melting temperature, or temperature at which the double-stranded DNA disassociates into its single-stranded components, is characteristic of the specific DNA sequence used and can be determined through temperature-dependent UV-visible absorption measurements.
The observed melting temperature of a DNA sequence can be sensitive to changes in the buffer concentration, the species present in solution, and the presence of base-pair mismatches within the sequence. As a result, the determination of a specific sequence’s melting temperature can be used as a diagnostic for changes in the anticipated sequence makeup or solvent environment. In this webinar, we discuss how to use UV-visible absorption spectroscopy to determine the melting temperature of double-stranded DNA in different environments, including sequences containing base-pair mismatches.
Watch this webinar to learn about these topics:
- How to use UV-visible absorption techniques to determine the melting temperature of double-stranded DDBA
- Understanding the environmental factors that can change the calculated melting temperature of a DNA sample
About the presenter
Dr. Jennifer Empey, Applications Scientist, UV-Vis Spectroscopy, Thermo Fisher Scientific
Jennifer Empey has been with Thermo Fisher Scientific as an Applications Scientist since October, 2021. She supports the Core UV-Visible instruments. Prior to this position, she received her PhD in Analytical Chemistry from The Ohio State University, where she used various spectroscopic techniques to study colloidal nanoparticles.
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