Spatial Biology Resource Center

Enable your multiplex imaging experiments with publications, protocols, and technical guidance


Spatial Biology is dedicated to understanding the location of biomarkers and cell types within tissues and organs. Our resource center is created by scientists to help start or evolve IHC experiments into multi-biomarker experiments. Get started with our educational videos, protocols, tips and tricks to enable your multiplex imaging experiments.

 

Scientific posters

Learn from our researchers the techniques and reagents to transform your imaging experiments into spatial phenotyping. 

Montoya 2024 AACR Poster

ThermoFisher Scientific provides a streamlined process for tissue labeling; a process that enables sufficient labeling with high plex panels completed within a couple of hours. We show successful detection of a wide range of various protein markers across numerous tissue organs used in various biological research applications with our uniform technique of labeling.

Clarke 2024 AACR Poster

A simplified workflow in FFPE tissue sections where ISH is detected using branched ssDNA signal amplification visible by brightfield  and fluorescence imaging combined with multiplexed IHC using directly labeled antibodies detected by fluorescence.

Webinars

Watch our virtual webinars and training sessions to share tips, tricks, and best practices on using our products. 

How to pick antibodies for spatial biology

Get information about biomarker panel design, staining protocols, antibody and clone choices, and data interpretation strategies for spatial biology research applications.

Introduction to harnessing single cell–resolution data

Watch this webinar for a primer for how to get started handling single-cell resolution data and discuss how powerful big data techniques can be for discovery.

Overcome common IHC problems

Discover easily incorporated tips to prevent sample autofluorescence, unspecific antibody labeling, fluorescent bleed-through and spectral overlap for usable IHC and spatial biology data.

Tissue preparations and handling for multiplex imaging in spatial biology research

Learn about tissue handling and fixation, epitope accessibility, and mounting needs for obtaining spatial biology investigations.

Solutions for your spatial biology experiment

Analyze single cells in spatial context with our imaging analysis products. Thermo Fisher Scientific offers high-quality reagents, assays, and instruments for experimental needs.

Protocols for a multiplex IHC experiment

These imaging protocols detail what you need for a successful multiplex IHC or spatial biology experiment, including tips, detailed steps, and lists of needed supplies. Learn how to use antibodies, common IHC supplies, labels, mountants, and counterstains to achieve stunning cellular

images. 

Multiplex IHC tyramide amplification protocol

Learn how to use multiple primary antibodies and tyramide signal amplification assays in this detailed protocol. Instructions on cyclic amplification are also available in the protocol. The advantage of this fluorescent staining is that primary antibodies of the same species can be used without the concern for crosstalk and the fluorophores generate bright multiplex signal. 

ReadyLabel antibody labeling assay protocol

Read this protocol and learn to label primary antibodies with Alexa Fluor dyes to form a stable, conjugated antibody. The benefit of using primary antibodies conjugated to fluorescent dyes includes being able to build over a 4 color-multiplex experiment without the issues of carefully choosing multiple antibodies from different species. 

ViewRNA ISH for tissue protocol

Understand the steps required to label multiple transcripts in a tissue sample with ViewRNA ISH Tissue Fluorescence Assay. Detecting transcripts within tissue is difficult and laborious. Learn how to choose the kits, prepare reagents, have the correct positive and negative controls, and perform the steps. 

Articles for a multiplex IHC experiment

Organ Mapping Antibody Panels (OMAPs)

Learn about picking multiple protein markers using OMAPs. This is a step-by-step guide to help navigate community resources to pick markers and antibodies.

Imaging Tools

Find helpful tools below to help you plan your imaging experiments. 

Resources and support

Photo credit, headline image: IBEX image of B cells in the germinal center and medulla of the human lymph node. Image is courtesy of Drs. Andrea J. Radtke and Ronald N. Germain.

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For Research Use Only. Not for use in diagnostic procedures.