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pSilencer™ 3.1-H1 neo
pSilencer™ 3.1-H1 neo Vector Map
This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.
pSilencer™ 3.1-H1 neo Annotated Promoter/Cloning Site Region
We recommend the following primers for sequencing:
M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'
3.0rev: 5'-GAGTTAGCTCACTCATTAGGC-3'
pSilencer™ 3.1-H1 neo Restriction Sites (Vector Size: 4285 bp)
Enzymes with single Restriction Sites
| Site | Enzyme |
|---|---|
| 4220 | AatII |
| 915 | AflIII |
| 1808 | AhdI |
| 1331 | AlwNI |
| 3772 | AvaI |
| 3793 | AvrII |
| 495 | BamHI |
| 3226 | BanII |
| 2313 | BcgI |
| 2347 | BcgI` |
| 3742 | BclI |
| 3283 | BsaAI |
| 3728 | BsaBI |
| 790 | BsaXI |
| 760 | BsaXI` |
| 3803 | BseRI |
| 45 | BsmBI |
| 2667 | EagI |
| 4274 | EcoO109I |
| 396 | EcoRI |
| 556 | HindIII |
| 3505 | MscI |
| 184 | NdeI |
| 2903 | NruI |
| 915 | PciI |
| 3538 | PstI |
| 3068 | RsrII |
| 2288 | ScaI |
| 4025 | SexAI |
| 3774 | SmaI |
| 3798 | StuI |
| 3468 | Tth111I |
| 3772 | XmaI |
| 2407 | XmnI |
| 4218 | ZraI |
Non-Cutting Enzymes:
| Enzyme |
|---|
| AarI |
| Acc65I |
| AccI |
| AfeI |
| AflII |
| AgeI |
| AleI |
| AloI |
| AloI' |
| ApaI |
| AscI |
| AsiSI |
| BaeI |
| BaeI` |
| BbsI |
| BbvCI |
| BglII |
| BlpI |
| BmgBI |
| BmtI |
| Bpu10I |
| BsgI |
| BsiWI |
| BspEI |
| BsrGI |
| BstBI |
| BstEII |
| BstXI |
| BstZ17I |
| Bsu36I |
| BtrI |
| ClaI |
| DraIII |
| EcoICRI |
| EcoNI |
| EcoRV |
| FseI |
| FspAI |
| HincII |
| HpaI |
| KpnI |
| MfeI |
| MluI |
| NheI |
| NotI |
| PacI |
| PflMI |
| PmeI |
| PmlI |
| PpuMI |
| PshAI |
| PspOMI |
| PsrI |
| PsrI` |
| SacI |
| SacII |
| SalI |
| SanDI |
| SbfI |
| SfiI |
| SgfI |
| SgrAI |
| SnaBI |
| SpeI |
| SrfI |
| SwaI |
| TaqII' |
| XbaI |
| XcmI |
| XhoI |