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Jurkat cells stained with CD3 antibody-PE-Cy®5.5 conjugate
Jurkat (human T-cell leukemia) cells were treated with 10 µM EdU for 2 hours, stained with CD3 mouse anti-human mAb PE-Cy®5.5 (Cat. No. MHCD0318) and detected according to the recommended staining protocol. The figures show a clear separation of proliferating cells which have incorporated EdU and nonproliferating cells which have not. Panel A shows data from cells labeled with Alexa Fluor® 488 picolyl azide analyzed on an Attune® Acoustic Focusing Cytometer using 488 nm excitation and a 530/30 nm bandpass emission filter; Panel B shows the same cells using 488 nm excitation and a 574/26 nm bandpass emission filter. The black outlined histogram is the cells stained with CD3 mouse anti-human mAb PE-Cy®5.5 and Click-iT® Plus EdU Alexa Fluor® 488 picolyl azide. The gray outlined histogram is theCD3 mouse anti-human mAb PE-Cy®5.5 positive control cells treated the same but without copper in the reaction.
