Proliferation of muntjac cells detected with Click-iT™ EdU.

Muntjac cells were treated with 10 µM EdU (Cat. No. A10044) for 45 min; cells were then fixed and permeabilized, and EdU that had been incorporated into newly synthesized DNA was detected using the far red–fluorescent Click-iT™ EdU Alexa Fluor® 647 High-Throughput (HCS) Assay (Cat. No. A10208), utilizing the technical tip for converting the HCS assay to conventional fluorescence microscopy. Tubulin was labeled with an anti-α-tubulin antibody (Cat. No. A11126) and visualized with Alexa Fluor® 350 Goat Anti–Mouse IgG (Cat. No. A11045, A21049). The Golgi complex was stained with green-fluorescent Alexa Fluor® 488 conjugate of lectin HPA from Helix pomatia (edible snail) (Cat. No. L11271), and peroxisomes were labeled with an anti-peroxisome antibody and visualized with orange-fluorescent Alexa Fluor® 555 Donkey Anti–Rabbit IgG (Cat. No. A31572).

Muntjac cells were treated with 10 µM EdU (Cat. No. A10044) for 45 min; cells were then fixed and permeabilized, and EdU that had been incorporated into newly synthesized DNA was detected using the far red–fluorescent Click-iT™ EdU Alexa Fluor® 647 High-Throughput (HCS) Assay (Cat. No. A10208), utilizing the technical tip for converting the HCS assay to conventional fluorescence microscopy. Tubulin was labeled with an anti-α-tubulin antibody (Cat. No. A11126) and visualized with Alexa Fluor® 350 Goat Anti–Mouse IgG (Cat. No. A11045, A21049). The Golgi complex was stained with green-fluorescent Alexa Fluor® 488 conjugate of lectin HPA from <i>Helix pomatia</i> (edible snail) (Cat. No. L11271), and peroxisomes were labeled with an anti-peroxisome antibody and visualized with orange-fluorescent Alexa Fluor® 555 Donkey Anti–Rabbit IgG (Cat. No. A31572).

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