NIH 3T3 cells showing incorporation of BrdU.

Cells were pulsed with BrdU (Cat. No. B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (Cat. No. A21300) and green-fluorescent Alexa Fluor® 488 Goat Anti–Mouse IgG1 isotype–specific secondary antibody (Cat. No. A21121); the BrdU staining pattern is co-localized with the nuclear staining of blue-fluorescent DAPI (Cat. No. D1306, D21490). Mitochondria were labeled with anti-COX, Complex IV, subunit I, and red-fluorescent Alexa Fluor® 594 Goat Anti–Mouse IgG2a isotype-specific antibody (Cat. No. A21135). Coverslips were mounted with Prolong® antifade mounting medium (Cat. No. P7481).

Cells were pulsed with BrdU (Cat. No. B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (Cat. No. A21300) and green-fluorescent Alexa Fluor® 488 Goat Anti–Mouse IgG1 isotype–specific secondary antibody (Cat. No. A21121); the BrdU staining pattern is co-localized with the nuclear staining of blue-fluorescent DAPI (Cat. No. D1306, D21490). Mitochondria were labeled with anti-COX, Complex IV, subunit I, and red-fluorescent Alexa Fluor® 594 Goat Anti–Mouse IgG2a isotype-specific antibody (Cat. No. A21135). Coverslips were mounted with Prolong® antifade mounting medium (Cat. No. P7481).

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货号 产品名称 规格 单价 (CNY) 数量
A21121 Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 500 µg
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A21135 Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 500 µg
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B23151 BrdU (5-Bromo-2'-Deoxyuridine) Each
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D21490 DAPI, FluoroPure™ grade, 10 mg, 10 mg Each
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