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Protein detection on microarrays using BODIPY® FL-X SE or SYPRO® Ruby protein blot stain.
Protein detection on microarrays using BODIPY® FL-X SE or SYPRO® Ruby protein blot stain. Bovine serum albumin was arrayed onto a PVDF membrane using a Packard BioScience PiezoTip dispenser. The array contains 72 spots with 12 dilutions of the dye, in replicates of 6, ranging from 666–0.325 pg per spot. The proteins were stained with either BODIPY® FL-X SE (D6102, left) in sodium borate buffer, pH 9.5, or with SYPRO® Ruby protein blot stain (S11791, right). Arrays were imaged on a ScanArray 5000XL microarray analysis system (Packard BioScience) and pseudocolored such that the different colors indicate different signal intensities. The sensitivity limit was 1.3–2.6 pg of protein in a 175 µm spot for the BODIPY® FL-X SE detection technique and 104 pg of protein per 175 µm spot for the SYPRO® Ruby protein blot stain technique.
