Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
产品信息
RBOIL4I
应用
建议稀释比
已发表文章
产品规格
种属
Bovine
Expression System
P. pastoris
氨基酸序列
aa 25-135
分子量
12.6 kDa
分类
Recombinant
类型
Protein
活性
ED50 = 0.44 - 0.67 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity of recombinant bovine IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line, TF-1 (Kitamura, T. et al. (1989) J. Cell Physiol. 140:323). The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 0.44 - 0.67 ng/mL.
Expression: Mature bovine IL-4 was expressed in Pichia pastoris, X-33. GenBank® Accession # P30367, amino acids 25 - 135. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLAEI.
Reconstitute with 0.5 mL of distilled water.
靶标信息
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: B-cell stimulatory factor 1; BSF-1; H-IL-4; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
基因别名: BSF-1; IL-4; IL4
UniProt ID: (Bovine) P30367
Entrez Gene ID: (Bovine) 280824
