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Human EGF Protein (PHG0311) in Functional
Immunofluorescence analysis of signal transducer and activator of transcription 3 (STAT3) was performed using A549 cells treated with 100 ng/mL human EGF recombinant protein (Product # PHG0311) for 30 minutes. The cells were labeled with phospho-STAT3 (Ser727) recombinant rabbit monoclonal antibody (Product # MA5-33199) (Panel a & c; Green). The composite (b & d) represents the merged with DAPI (blue) and F... View More
产品信息
PHG0311
应用
建议稀释比
已发表文章
产品规格
种属
Human
已发表种属
Human
Expression System
E. coli
分子量
6.2 kDa
分类
Recombinant
类型
Protein
纯度
>95% by SDS-PAGE
内毒素浓度
<0.1 ng/µg
活性
ED50 < 0.4 ng/mL; determined by the dose-dependent proliferation of mouse BALB/3T3 cells.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
Carrier-Free
Reconstitution: Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, PBS buffer to a concentration of 0.1-1.0 mg/mL. Make any further dilutions of the reconstituted protein in low endotoxin medium, or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA
Storage: Store the lyophilized protein at 2°C to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
靶标信息
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: beta-urogastrone; EGF; H-EGF; Pro-epidermal growth factor
基因别名: EGF; HOMG4; URG
UniProt ID: (Human) P01133
Entrez Gene ID: (Human) 1950
