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产品信息
A42534
应用
建议稀释比
已发表文章
产品规格
种属
Human
Expression System
CHO cells
氨基酸序列
Human CD16a, amino acids Met1-Gln208 (Accession # P08637-1) with natural variant F176V with a C-terminal His-tag
标签
His-tag
分子量
23.3 kDa
分类
Recombinant
类型
Protein
纯度
≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
内毒素浓度
<1.0 EU/µg
活性
Measured by its binding ability in a functional ELISA. Immobilized Human Fc gamma RIIIA (CD16a) (176 Val) His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind human IgG1, the EC50 of human IgG1 is 200-600 ng/mL.
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS, pH 7.2, with 5% mannitol, 5% trehalose, 0.01% Tween 80
内含物
no preservative
保存条件
-20°C or -80°C if preferred
运输条件
Ambient
产品详细信息
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
靶标信息
CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32 receptor 2; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1
基因别名: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20
UniProt ID: (Human) P08637, (Human) O75015
Entrez Gene ID: (Human) 2214, (Human) 2215
