Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
产品信息
200-33-10UG
应用
建议稀释比
已发表文章
产品规格
种属
Human
已发表种属
Human,
Mouse
Expression System
E. coli
氨基酸序列
SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET
分子量
17.9 kDa
分类
Recombinant
类型
Protein
纯度
≥ 98% by SDS-PAGE gel and HPLC analyses.
内毒素浓度
<1 EU/µg
活性
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine D10S cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
产品详细信息
Recombinant Human IL-33 is a 17.9 kDa protein containing 159 amino acid residues.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
靶标信息
IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DVS27-related protein; growth stimulation-expressed; H-IL-33; homolog of mouse growth stimulation-expressed; IL-1F11; IL-33; ILN; Interleukin; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin-1 family member 11; Interleukin-1 receptor-like 1; Interleukin-33; Interleukin33; NF-HEV; nuclear factor for high endothelial venules; Nuclear factor from high endothelial venules; Protein ST2; RP11-575C20.2
基因别名: C9orf26; DER4; DVS27; FIT-1; IL1F11; IL1RL1; IL33; IL33R; NF-HEV; NFEHEV; NFHEV; ST2; ST2L; ST2V; T1
UniProt ID: (Human) Q01638, (Human) O95760
Entrez Gene ID: (Human) 9173, (Human) 90865
