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产品信息
PHP0013
应用
建议稀释比
已发表文章
产品规格
种属
Human
Expression System
E. coli
氨基酸序列
Human Leptin recombinant protein contains 147 amino acids
分子量
16 kDa
分类
Recombinant
类型
Protein
纯度
>98% by SDS-PAGE
内毒素浓度
<0.1 ng/µg
活性
Determined active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed compared to saline-treated controls.
偶联物
形式
Lyophilized
纯化类型
purified
保存液
0.1% TFA
内含物
no preservative
保存条件
-20°C
运输条件
Wet ice (domestic); Wet or Dry ice (international)
产品详细信息
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute leptin in sterile, distilled water to a concentration of 1.0-5.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
靶标信息
Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD295 antigen; H-Leptin; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Obese protein; obese, mouse, homolog of; obesity; Obesity factor; obesity protein
基因别名: LEP; LEPD; OB; OBS
UniProt ID: (Human) P41159
Entrez Gene ID: (Human) 3952
