Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
产品信息
PHC9501
应用
建议稀释比
已发表文章
产品规格
种属
Human
已发表种属
Human,
Mouse
Expression System
HEK293 cells
分子量
38 kDa
分类
Recombinant
类型
Protein
纯度
>95% by SDS-PAGE
内毒素浓度
<0.1 ng/µg
活性
ED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of M-NSF60 cells.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
Reconstitution: Lyophilized human M-CSF should be reconstituted in 20mM Tris-HCl, pH 8.0 to a concentration of 0.1-1.0 mg/mL. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: Lyophilized human M-CSF should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted human M-CSF at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.
靶标信息
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: colony stimulating factor 1 (macrophage); CSF-1; H-MCSF; Lanimostim; M-MCSF; macrophage colony stimulating factor 1; Macrophage colony-stimulating factor 1; MGC31930; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor
基因别名: CSF-1; CSF1; MCSF
UniProt ID: (Human) P09603
Entrez Gene ID: (Human) 1435
