Gibco

Human/Mouse/Rat Myostatin Recombinant Protein, PeproTech®

Human/Mouse/Rat Myostatin Protein in Functional Assay (Functional)

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Human/Mouse/Rat Myostatin Protein (120-00-10UG) in Functional

Bioassay analysis of Human/Mouse/Rat Myostatin Recombinant Protein, PeproTech® (Product # 120-00-1MG).

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产品信息

120-00-10UG

应用

建议稀释比

已发表文章

功能检测 (Functional)

Assay-dependent

点杂交 (DB)

查看1篇已发表文章 1篇已发表文章

体外检测 (IV)

查看7篇已发表文章 7篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属

Human, Mouse, Rat

已发表种属

Human, Mouse

Expression System

E. coli

氨基酸序列

DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS

分子量

25 kDa

分类

Recombinant

类型

Protein

纯度

≥ 98% by SDS-PAGE gel and HPLC analyses.

内毒素浓度

<1 EU/µg

活性

Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is 17.0-25.0 ng/ml.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

purified

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

产品详细信息

The amino acid sequence of mature myostatin is extremely conserved across species, and is the same in murine, rat, chicken, turkey, porcine, and human. Myostatin is expressed as the C-terminal part of a precursor polypeptide, which also contains a short N-terminal signal sequence for secretion, and a propeptide of 243 amino acids. After dimerization of this precursor, the covalent bonds between the propeptide and the mature ligand are cleaved by furin-type proteases. However, the resulting two proteins remain associated through non-covalent interactions, and are secreted as a latent complex.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

靶标信息

The protein encoded by this gene is a member of the bone morphogenetic protein family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Differentiation of stem cell-derived pancreatic progenitors into insulin-secreting islet clusters in a multiwell-based static 3D culture system.

Cell reports methods

Liang S,Zhao J,Baker RK,Tran E,Zhan L,Kieffer TJ

120-00 was used in Cell Culture to describe a 96-well-based static suspension culture method for differentiation of pancreatic progenitors into human pluripotent stem cell (hPSC)-islets.

Mon May 22 00:00:00 EDT 2023

Non-xenogeneic expansion and definitive endoderm differentiation of human pluripotent stem cells in an automated bioreactor.

Biotechnology and bioengineering

Jacobson EF,Chen Z,Stoukides DM,Nair GG,Hebrok M,Tzanakakis ES

120-00 was used in In vitro experiments to demonstrate the xeno-free expansion and directed differentiation of human embryonic and induced pluripotent stem cells to definitive endoderm (DE) in a controlled stirred suspension bioreactor (SSB).

Mon Feb 01 00:00:00 EST 2021

Enhanced myostatin expression and signalling promote tubulointerstitial inflammation in diabetic nephropathy.

Scientific reports

Verzola D,Milanesi S,Viazzi F,Ansaldo F,Saio M,Garibaldi S,Carta A,Costigliolo F,Salvidio G,Barisione C,Esposito P,Garibotto G,Picciotto D

Tue Apr 14 00:00:00 EDT 2020

Exogenous GDF11, but not GDF8, reduces body weight and improves glucose homeostasis in mice.

Scientific reports

Walker RG,Barrandon O,Poggioli T,Dagdeviren S,Carroll SH,Mills MJ,Mendello KR,Gomez Y,Loffredo FS,Pancoast JR,Macias-Trevino C,Marts C,LeClair KB,Noh HL,Kim T,Banks AS,Kim JK,Cohen DE,Wagers AJ,Melton DA,Lee RT

Thu Mar 12 00:00:00 EDT 2020

MyoScreen, a High-Throughput Phenotypic Screening Platform Enabling Muscle Drug Discovery.

SLAS discovery : advancing life sciences R & D

Young J,Margaron Y,Fernandes M,Duchemin-Pelletier E,Michaud J,Flaender M,Lorintiu O,Degot S,Poydenot P

120-00 was used in In vitro experiments to investigate MyoScreen treatment of muscle atrophy and disease.

Sat Sep 01 00:00:00 EDT 2018

Growth Differentiation Factor 11 treatment leads to neuronal and vascular improvements in the hippocampus of aged mice.

Scientific reports

Ozek C,Krolewski RC,Buchanan SM,Rubin LL

Fri Nov 23 00:00:00 EST 2018

Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer.

Developmental cell

Bajikar SS,Wang CC,Borten MA,Pereira EJ,Atkins KA,Janes KA

120-00 was used in Dot blotting to study the tumour-suppressive mode of action of growth-differentiation factor 11, and an unusual mechanism of its inactivation in triple negative breast cancer.