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产品信息
PV4046
产品规格
种属
Human
Expression System
Insect cells
氨基酸序列
306-648; Y340D, Y341D
标签
GST-tag
分子量
66.4 kDa
分类
Recombinant
类型
Protein
纯度
80% by SDS-PAGE
偶联物
形式
Liquid
浓度
See Label
纯化类型
purified
保存液
50mM tris, pH 7.5, with 2mM DTT, 150mM NaCl, 50% glycerol, 0.5mM EDTA, 0.02% Triton X-100
内含物
no preservative
保存条件
-80° C, Avoid Freeze/Thaw Cycles
运输条件
Dry ice
产品详细信息
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Protein Form: Recombinant, Catalytic, Mutant
靶标信息
RAF1 (cRAF) is a MAP kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylates and activates the serine/threonine specific protein kinases ERK1 and ERK2. The RAF1 Y340D Y341D mutant is constitutively active. RAF1 functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
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