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Human VEGF-165 Protein (RVEGFI) in Functional
Cell proliferation assay human VEGF-stimulated HUVEC cells was performed by resuspending HUVEC cells in Earle Salts (199X without h-EGF) containing 10% heat-inactivated FBS (assay media). Five thousand cells per well (corresponding to 100 µL/well, passage 1) were transferred to an assay plate and 100 µL/well of human VEGF (Product # RVEGFI) was serially diluted 1:3 in triplicate, from a starting concentrati... View More
产品信息
RVEGFI
应用
建议稀释比
已发表文章
产品规格
种属
Human
已发表种属
Not Applicable
Expression System
P. pastoris
氨基酸序列
Human VEGF-165, amino acids 27-191 (Accession # 003376)
分子量
19.2 kDa
分类
Recombinant
类型
Protein
纯度
>95% by SDS-PAGE
活性
Determined by the proliferation effect on human umbilical vein endothelial cells (HUVECs).
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
Mature human VEGF165 was expressed in Pichia pastoris, KM71. GenBank Accession # 003376, amino acids 27 - 191. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: EGGGQNHHEV. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant human VEGF165 was determined by measuring the proliferation of human umbilical vein endothelial cells (HUVEC).
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4ºC. Reconstitute in 1 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70ºC, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
靶标信息
The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: H-VEGF-165; L-VEGF; M-VEGF-165; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; VEGF165; VPF
基因别名: MVCD1; VEGF; VEGFA; VPF
UniProt ID: (Human) H3BLW7
Entrez Gene ID: (Human) 7422
