Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
产品信息
PMG0031
应用
建议稀释比
已发表文章
产品规格
种属
Mouse
Expression System
E. coli
氨基酸序列
PALPEDGGAA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
分子量
17 kDa
分类
Recombinant
类型
Protein
纯度
>95% by SDS-PAGE
内毒素浓度
<0.1 ng/µg
活性
ED50 = 0.1 - 1.0 ng/mL; determined by the dose-dependent proliferation of BALB/3T3 cells.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized mouse FGFb in sterile, distilled water to 0.1-0.5 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilution should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA. Storage: Lyophilized mouse FGFb should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted mouse FGFb at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.
靶标信息
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: Basic fibroblast growth factor; bFGF; FGF; fgf basic; FGF-2; FGF-b; Fibroblast growth factor; Fibroblast growth factor 2; H-FGF-b-147; H-FGF-b-154; HBGF-2; Heparin-binding growth factor 2; M-FGF-b
基因别名: bFGF; Fgf-2; Fgf2; Fgfb
UniProt ID: (Mouse) P15655
Entrez Gene ID: (Mouse) 14173
