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Mouse IL-22 Recombinant Protein, PeproTech®

Mouse IL-22 Protein in Functional Assay (Functional)

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Mouse IL-22 Protein (210-22-10UG) in Functional

Bioassay analysis of Mouse IL-22 Recombinant Protein, PeproTech® (Product # 210-22-1MG).

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产品信息

210-22-10UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

查看3篇已发表文章 3篇已发表文章

功能检测 (Functional)

Assay-dependent

T细胞活化 (TCA)

查看1篇已发表文章 1篇已发表文章

体外检测 (IV)

查看14篇已发表文章 14篇已发表文章

其他PubMed (Misc)

查看8篇已发表文章 8篇已发表文章

产品规格

种属

Mouse

已发表种属

Human, Mouse

Expression System

E. coli

氨基酸序列

MLPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV

分子量

33.4 kDa

分类

Recombinant

类型

Protein

纯度

≥ 98% by SDS-PAGE gel and HPLC analyses.

内毒素浓度

<1 EU/µg

活性

Determined by its ability to induce STAT3 activation in COLO 205 cells. The expected ED50 for this effect is 150-400 pg/ml.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

purified

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

产品详细信息

Recombinant Murine IL-22 is a 33.4 kDa, non-disulfide-linked, homodimeric protein consisting of two 147 amino acid polypeptide chains.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

靶标信息

IL-22 also known as IL-10-related T-cell derived inducible factor, is an alpha helical cytokine and is considered a member of the IFN-IL-10 family, which includes IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and the type I and II interferons. IL-22 is produced mainly by activated T cells and NK cells. In humans, the IL-22 gene is located on the q arm of chromosome 12, and is structurally related to IL10. IL-22 acts by engaging the heterodimeric receptor complex consisting of primary receptor IL-22R1 and accessory receptor IL-10R2. IL-22R1 also binds IL-20 and IL-24; IL-10R2 also binds IL-10, IL-27, IL-28, and IL-29. Binding of IL-22 to its receptor complex induces signal transduction, particularly via the JAK-STAT pathway. In addition to the membrane-bound IL-22R1/IL-10R2 complex, a soluble single-chain IL-22 receptor termed IL-22BP has been found to antagonize IL-22 binding and signaling. IL-22 appears not to directly influence immune cells, and major targets of the cytokine appear to be nonimmune cells, such as cells of the skin, digestive and respiratory system, as well as hepatocytes, and keratinocytes. IL-22 has been described as an effector cytokine of the Th17 lineage. Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin. IL-17A, IL-17F and IL-22 are all co-expressed by Th17 cells, however, they are differentially regulated. The effects of IL-22 include induction of acute phase reactants and antimicrobial proteins, as well as increasing the mobility of keratinocytes. IL-22 is highly expressed during chronic inflammation, and found to activate intracellular kinases and transcription factors. IL-22 is critical for host defense against infections of extracellular pathogens, and promotes wound-healing responses. IL-22 is upregulated in activated T cells. IL-22 has been reported to mediate IL-23-induced acanthosis and dermal inflammation through activation of STAT3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasis.

Immunity

Briukhovetska D,Suarez-Gosalvez J,Voigt C,Markota A,Giannou AD,Schübel M,Jobst J,Zhang T,Dörr J,Märkl F,Majed L,Müller PJ,May P,Gottschlich A,Tokarew N,Lücke J,Oner A,Schwerdtfeger M,Andreu-Sanz D,Grünmeier R,Seifert M,Michaelides S,Hristov M,König LM,Cadilha BL,Mikhaylov O,Anders HJ,Rothenfusser S,Flavell RA,Cerezo-Wallis D,Tejedo C,Soengas MS,Bald T,Huber S,Endres S,Kobold S

210-22 was used in Cell Culture to reveal an immunosuppressive circuit activated by T cell-derived IL-22 that promotes lung metastasis.

Tue Jan 10 00:00:00 EST 2023

Active eosinophils regulate host defence and immune responses in colitis.

Nature

Gurtner A,Borrelli C,Gonzalez-Perez I,Bach K,Acar IE,Núñez NG,Crepaz D,Handler K,Vu VP,Lafzi A,Stirm K,Raju D,Gschwend J,Basler K,Schneider C,Slack E,Valenta T,Becher B,Krebs P,Moor AE,Arnold IC

210-22 was used in In vitro experiments to provide insights into the biology of eosinophils and highlight the crucial contribution of this cell type to intestinal homeostasis, immune regulation and host defence.

Wed Mar 01 00:00:00 EST 2023

Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation.

Cell stem cell

He GW,Lin L,DeMartino J,Zheng X,Staliarova N,Dayton T,Begthel H,van de Wetering WJ,Bodewes E,van Zon J,Tans S,Lopez-Iglesias C,Peters PJ,Wu W,Kotlarz D,Klein C,Margaritis T,Holstege F,Clevers H

210-22 was used in Tissue/organ culture to optimise human small intestinal organoid culturing, constitutively generating all differentiated cell types while maintaining an active stem cell compartment.

Thu Sep 01 00:00:00 EDT 2022

Optic nerve regeneration screen identifies multiple genes restricting adult neural repair.

Cell reports

Lindborg JA,Tran NM,Chenette DM,DeLuca K,Foli Y,Kannan R,Sekine Y,Wang X,Wollan M,Kim IJ,Sanes JR,Strittmatter SM

Tue Mar 02 00:00:00 EST 2021

Synergistic effect of the TLR5 agonist CBLB502 and its downstream effector IL-22 against liver injury.

Cell death & disease

Melin N,Sánchez-Taltavull D,Fahrner R,Keogh A,Dosch M,Büchi I,Zimmer Y,Medová M,Beldi G,Aebersold DM,Candinas D,Stroka D

Tue Apr 06 00:00:00 EDT 2021

Interferon-γ inhibits sirtuin 6 gene expression in intestinal epithelial cells through a microRNA-92b-dependent mechanism.

American journal of physiology. Cell physiology

Liu F,Wang X,Geng H,Bu HF,Wang P,De Plaen IG,Yang H,Qian J,Tan XD

Wed Apr 01 00:00:00 EDT 2020

A Central Role for Lipocalin-2 in the Adaptation to Short-Bowel Syndrome Through Down-Regulation of IL22 in Mice.

Cellular and molecular gastroenterology and hepatology

Zhang A,Sodhi CP,Wang M,Shores DR,Fulton W,Prindle T,Brosten S,O'Hare E,Lau A,Ding H,Jia H,Lu P,White JR,Hui J,Sears CL,Hackam DJ,Alaish SM

210-22 was used in Sample Prep (PCR) to lCN2 promotes inflammation and slows intestinal adaptation through changes in the microbiome and IL22 inhibition in a mouse SBS model.

Fri Jul 09 00:00:00 EDT 2021

A Central Role for Lipocalin-2 in the Adaptation to Short-Bowel Syndrome Through Down-Regulation of IL22 in Mice.

Cellular and molecular gastroenterology and hepatology

Zhang A,Sodhi CP,Wang M,Shores DR,Fulton W,Prindle T,Brosten S,O'Hare E,Lau A,Ding H,Jia H,Lu P,White JR,Hui J,Sears CL,Hackam DJ,Alaish SM

210-22 was used in Sample Prep (PCR) to lCN2 promotes inflammation and slows intestinal adaptation through changes in the microbiome and IL22 inhibition in a mouse SBS model.

Fri Jul 09 00:00:00 EDT 2021

Dietary lipids fuel GPX4-restricted enteritis resembling Crohn's disease.

Nature communications

Mayr L,Grabherr F,Schwärzler J,Reitmeier I,Sommer F,Gehmacher T,Niederreiter L,He GW,Ruder B,Kunz KTR,Tymoszuk P,Hilbe R,Haschka D,Feistritzer C,Gerner RR,Enrich B,Przysiecki N,Seifert M,Keller MA,Oberhuber G,Sprung S,Ran Q,Koch R,Effenberger M,Tancevski I,Zoller H,Moschen AR,Weiss G,Becker C,Rosenstiel P,Kaser A,Tilg H,Adolph TE

210-22 was used in Cell activation to identify dietary polyunsaturated fatty acids (PUFAs) as a trigger of GPX4-restricted mucosal inflammation phenocopying aspects of human Crohn's disease (CD).

Tue Apr 14 00:00:00 EDT 2020

IL-22 ameliorates LPS-induced acute liver injury by autophagy activation through ATF4-ATG7 signaling.

Cell death & disease

Shao L,Xiong X,Zhang Y,Miao H,Ren Y,Tang X,Song J,Wang C

Wed Nov 11 00:00:00 EST 2020

ATG16L1 orchestrates interleukin-22 signaling in the intestinal epithelium via cGAS-STING.

The Journal of experimental medicine

Aden K,Tran F,Ito G,Sheibani-Tezerji R,Lipinski S,Kuiper JW,Tschurtschenthaler M,Saveljeva S,Bhattacharyya J,Häsler R,Bartsch K,Luzius A,Jentzsch M,Falk-Paulsen M,Stengel ST,Welz L,Schwarzer R,Rabe B,Barchet W,Krautwald S,Hartmann G,Pasparakis M,Blumberg RS,Schreiber S,Kaser A,Rosenstiel P

Mon Nov 05 00:00:00 EST 2018

Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis.

Cell host & microbe

Zhu F,Willette-Brown J,Song NY,Lomada D,Song Y,Xue L,Gray Z,Zhao Z,Davis SR,Sun Z,Zhang P,Wu X,Zhan Q,Richie ER,Hu Y

210-22 was used in Enzyme-linked immunosorbent assay to demonstrate that autoreactive T-cells and chronic fungal infection, fostered by inflammation and epithelial injury, promote esophageal squamous cell carcinoma development.

Wed Apr 12 00:00:00 EDT 2017

IL-22 Upregulates Epithelial Claudin-2 to Drive Diarrhea and Enteric Pathogen Clearance.

Cell host & microbe

Tsai PY,Zhang B,He WQ,Zha JM,Odenwald MA,Singh G,Tamura A,Shen L,Sailer A,Yeruva S,Kuo WT,Fu YX,Tsukita S,Turner JR

210-22 was used in Cell Culture to investigate the pathogenic effects of diarrhea.

Wed Jun 14 00:00:00 EDT 2017

IL-22BP dictates characteristics of Peyer's patch follicle-associated epithelium for antigen uptake.

The Journal of experimental medicine

Jinnohara T,Kanaya T,Hase K,Sakakibara S,Kato T,Tachibana N,Sasaki T,Hashimoto Y,Sato T,Watarai H,Kunisawa J,Shibata N,Williams IR,Kiyono H,Ohno H

Mon Jun 05 00:00:00 EDT 2017

IL-22BP dictates characteristics of Peyer's patch follicle-associated epithelium for antigen uptake.

The Journal of experimental medicine

Jinnohara T,Kanaya T,Hase K,Sakakibara S,Kato T,Tachibana N,Sasaki T,Hashimoto Y,Sato T,Watarai H,Kunisawa J,Shibata N,Williams IR,Kiyono H,Ohno H

Mon Jun 05 00:00:00 EDT 2017

A Protective Function of IL-22BP in Ischemia Reperfusion and Acetaminophen-Induced Liver Injury.

Journal of immunology (Baltimore, Md. : 1950)

Kleinschmidt D,Giannou AD,McGee HM,Kempski J,Steglich B,Huber FJ,Ernst TM,Shiri AM,Wegscheid C,Tasika E,Hübener P,Huber P,Bedke T,Steffens N,Agalioti T,Fuchs T,Noll J,Lotter H,Tiegs G,Lohse AW,Axelrod JH,Galun E,Flavell RA,Gagliani N,Huber S

Fri Dec 15 00:00:00 EST 2017

IL-6 Signaling Regulates Small Intestinal Crypt Homeostasis.

Journal of immunology (Baltimore, Md. : 1950)

Jeffery V,Goldson AJ,Dainty JR,Chieppa M,Sobolewski A

Sat Jul 01 00:00:00 EDT 2017

IL-22 dampens the T cell response in experimental malaria.

Scientific reports

Sellau J,Alvarado CF,Hoenow S,Mackroth MS,Kleinschmidt D,Huber S,Jacobs T

Fri Jun 17 00:00:00 EDT 2016

IL-22 regulates lymphoid chemokine production and assembly of tertiary lymphoid organs.

Proceedings of the National Academy of Sciences of the United States of America

Barone F,Nayar S,Campos J,Cloake T,Withers DR,Toellner KM,Zhang Y,Fouser L,Fisher B,Bowman S,Rangel-Moreno J,Garcia-Hernandez Mde L,Randall TD,Lucchesi D,Bombardieri M,Pitzalis C,Luther SA,Buckley CD

Tue Sep 01 00:00:00 EDT 2015

Proliferation conditions promote intrinsic changes in NK cells for an IL-10 response.

Journal of immunology (Baltimore, Md. : 1950)

Tarrio ML,Lee SH,Fragoso MF,Sun HW,Kanno Y,O'Shea JJ,Biron CA

Tue Jul 01 00:00:00 EDT 2014

IL-22 mediates host defense against an intestinal intracellular parasite in the absence of IFN-γ at the cost of Th17-driven immunopathology.

Journal of immunology (Baltimore, Md. : 1950)

Stange J,Hepworth MR,Rausch S,Zajic L,Kühl AA,Uyttenhove C,Renauld JC,Hartmann S,Lucius R

Thu Mar 01 00:00:00 EST 2012

Interleukin-22 is produced by invariant natural killer T lymphocytes during influenza A virus infection: potential role in protection against lung epithelial damages.

The Journal of biological chemistry

Paget C,Ivanov S,Fontaine J,Renneson J,Blanc F,Pichavant M,Dumoutier L,Ryffel B,Renauld JC,Gosset P,Gosset P,Si-Tahar M,Faveeuw C,Trottein F

Fri Mar 16 00:00:00 EDT 2012

Cutting edge: crucial role of IL-1 and IL-23 in the innate IL-17 response of peripheral lymph node NK1.1- invariant NKT cells to bacteria.

Journal of immunology (Baltimore, Md. : 1950)

Doisne JM,Soulard V,Bécourt C,Amniai L,Henrot P,Havenar-Daughton C,Blanchet C,Zitvogel L,Ryffel B,Cavaillon JM,Marie JC,Couillin I,Benlagha K

Sat Jan 15 00:00:00 EST 2011

IL-22 production is regulated by IL-23 during Listeria monocytogenes infection but is not required for bacterial clearance or tissue protection.

PloS one

Graham AC,Carr KD,Sieve AN,Indramohan M,Break TJ,Berg RE

Tue Feb 15 00:00:00 EST 2011

Notch signaling drives IL-22 secretion in CD4+ T cells by stimulating the aryl hydrocarbon receptor.

Proceedings of the National Academy of Sciences of the United States of America

Alam MS,Maekawa Y,Kitamura A,Tanigaki K,Yoshimoto T,Kishihara K,Yasutomo K

Tue Mar 30 00:00:00 EDT 2010

IL-23 promotes maintenance but not commitment to the Th17 lineage.

Journal of immunology (Baltimore, Md. : 1950)

Stritesky GL,Yeh N,Kaplan MH

Sat Nov 01 00:00:00 EDT 2008

IL-22 is expressed by Th17 cells in an IL-23-dependent fashion, but not required for the development of autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Kreymborg K,Etzensperger R,Dumoutier L,Haak S,Rebollo A,Buch T,Heppner FL,Renauld JC,Becher B

Sat Dec 15 00:00:00 EST 2007

生物信息学

蛋白别名: il 22; IL-10-related T-cell-derived-inducible factor; IL-22; IL-22a; IL-TIF; IL-TIF alpha; ILN; ILTIF alpha; Interleukin; interleukin 10-related T cell-derived inducible factor; Interleukin-22; Interleukin-22a; Interleukin22

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基因别名: IL-22; IL-22a; Il22; Il22a; Iltif; ILTIFa

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UniProt ID: (Mouse) Q9JJY9

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Entrez Gene ID: (Mouse) 50929

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Mouse IL-22 Recombinant Protein, PeproTech®

Mouse IL-22 Protein (210-22-10UG) in Functional

产品信息

210-22-10UG

免疫印迹 (WB)

酶联免疫吸附实验 (ELISA)

功能检测 (Functional)

T细胞活化 (TCA)

体外检测 (IV)

其他PubMed (Misc)

种属

已发表种属

Expression System

氨基酸序列

分子量

分类

类型

纯度

内毒素浓度

活性

偶联物

形式

纯化类型

内含物

保存条件

运输条件

产品详细信息

靶标信息

生物信息学

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