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PeproGMP® Human EGF Recombinant Protein, PeproTech®

PeproGMP® Human EGF Protein in Functional Assay (Functional)

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PeproGMP® Human EGF Protein (GMP100-15-100UG) in Functional

Cell proliferation assay analysis of PeproGMP® Human EGF Recombinant Protein, PeproTech (Product # GMP100-15-100UG) compared to Human EGF, Animal-Free Recombinant Protein, PeproTech (Product # AF-100-15-1MG), demonstrating comparable bioactivity across product grades.

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产品信息

GMP100-15-100UG

应用

建议稀释比

已发表文章

功能检测 (Functional)

Assay-dependent

体外检测 (IV)

查看7篇已发表文章 7篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属

Human

已发表种属

Human, Mouse

Expression System

E. coli

氨基酸序列

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

分子量

6.2 kDa

分类

Recombinant

类型

Protein

纯度

≥ 98% by SDS-PAGE gel and HPLC analyses.

内毒素浓度

<0.1 EU/µg

活性

Determined by the proliferation of mouse Balb/c 3T3 cells. PeproGMP Recombinant Human EGF has been tested against the WHO standard NIBSC 91/530. Find the International Unit (IU) value here.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

purified

内含物

no preservative

保存条件

-20°C or -80°C if preferred

运输条件

Ambient

产品详细信息

Additional product information and specifications are provided on the Certificate of Analysis.

All PeproGMP® products are manufactured using animal-derived component free materials.

Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.

Product use statement: For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.

靶标信息

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Light and electron microscopy continuum-resolution imaging of 3D cell cultures.

Developmental cell

D'Imprima E,Garcia Montero M,Gawrzak S,Ronchi P,Zagoriy I,Schwab Y,Jechlinger M,Mahamid J

GMP100-15 was used in Cell Culture to demonstrate this workflow on mouse and human 3D cultures and use automated image segmentation to annotate and quantitatively analyze subcellular structures in patient-derived colorectal cancer organoids.

Mon Apr 10 00:00:00 EDT 2023

Programming inactive RNA-binding small molecules into bioactive degraders.

Nature

Tong Y,Lee Y,Liu X,Childs-Disney JL,Suresh BM,Benhamou RI,Yang C,Li W,Costales MG,Haniff HS,Sievers S,Abegg D,Wegner T,Paulisch TO,Lekah E,Grefe M,Crynen G,Van Meter M,Wang T,Gibaut QMR,Cleveland JL,Adibekian A,Glorius F,Waldmann H,Disney MD

GMP100-15 was used in Cell Culture to provide a proof of concept, designing selective degraders for the precursor to the disease-associated microRNA-155 (pre-miR-155), JUN mRNA and MYC mRNA.

Thu Jun 01 00:00:00 EDT 2023

Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression.

Autophagy

Xiao W,Wang J,Wang X,Cai S,Guo Y,Ye L,Li D,Hu A,Jin S,Yuan B,Zhou Y,Li Q,Tong Q,Zheng L

GMP100-15 was used in Cell Culture to indicate that therapeutic targeting of the ubiquitin specific peptidase 2-E2F transcription factor 4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression.

Tue Nov 01 00:00:00 EDT 2022

Nuclear stabilization of p53 requires a functional nucleolar surveillance pathway.

Cell reports

Hannan KM,Soo P,Wong MS,Lee JK,Hein N,Poh P,Wysoke KD,Williams TD,Montellese C,Smith LK,Al-Obaidi SJ,Núñez-Villacís L,Pavy M,He JS,Parsons KM,Loring KE,Morrison T,Diesch J,Burgio G,Ferreira R,Feng ZP,Gould CM,Madhamshettiwar PB,Flygare J,Gonda TJ,Simpson KJ,Kutay U,Pearson RB,Engel C,Watkins NJ,Hannan RD,George AJ

GMP100-15 was used in Cell Culture to demonstrate the ribosome biogenesis axis as the most potent class of genes whose disruption stabilizes p53.

Tue Nov 01 00:00:00 EDT 2022

Architectural control of metabolic plasticity in epithelial cancer cells.

Communications biology

Al-Masri M,Paliotti K,Tran R,Halaoui R,Lelarge V,Chatterjee S,Wang LT,Moraes C,McCaffrey L

Fri Mar 19 00:00:00 EDT 2021

Architectural control of metabolic plasticity in epithelial cancer cells.

Communications biology

Al-Masri M,Paliotti K,Tran R,Halaoui R,Lelarge V,Chatterjee S,Wang LT,Moraes C,McCaffrey L

Fri Mar 19 00:00:00 EDT 2021

Hypoxia-induced myeloid derived growth factor promotes hepatocellular carcinoma progression through remodeling tumor microenvironment.

Theranostics

Wang X,Mao J,Zhou T,Chen X,Tu H,Ma J,Li Y,Ding Y,Yang Y,Wu H,Tang X

GMP100-15 was used in Cell Culture to study whether myeloid derived growth factor (MYDGF) could accelerate the progression of hepatocellular carcinoma (HCC) and how it works to show that MYDGF could directly affect the self-renewal of liver cells, and indirectly aggravate the inflammatory microenvironment to accelerate the progression of HCC.

Thu Aug 05 00:00:00 EDT 2021

Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages.

Cancers

Wang YY,Chen HD,Lo S,Chen YK,Huang YC,Hu SC,Hsieh YC,Hung AC,Hou MF,Yuan SF

GMP100-15 was used in cell culture experiments to demonstrate that visfatin enhanced breast cancer progression via pERK/CXCL1 induction in macrophages.

Thu Nov 26 00:00:00 EST 2020

Bromodomain inhibitor jq1 induces cell cycle arrest and apoptosis of glioma stem cells through the VEGF/PI3K/AKT signaling pathway.

International journal of oncology

Wen N,Guo B,Zheng H,Xu L,Liang H,Wang Q,Wang D,Chen X,Zhang S,Li Y,Zhang L

GMP100-15 was used in Cell Culture to explore the underlying mechanisms of the anti-tumour effects of Brd4 and the bromodomain inhibitor JQ1 on glioma stem cells.

Tue Oct 01 00:00:00 EDT 2019

Endoplasmic reticulum stress disrupts lysosomal homeostasis and induces blockade of autophagic flux in human trophoblasts.

Scientific reports

Nakashima A,Cheng SB,Kusabiraki T,Motomura K,Aoki A,Ushijima A,Ono Y,Tsuda S,Shima T,Yoshino O,Sago H,Matsumoto K,Sharma S,Saito S

GMP100-15 was used in Cell Culture to show that ER stress reduces the number of lysosomes, resulting in inhibition of autophagic flux in trophoblast cells.

Wed Aug 07 00:00:00 EDT 2019

生物信息学

蛋白别名: beta-urogastrone; EGF; H-EGF; Pro-epidermal growth factor

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基因别名: EGF; HOMG4; URG

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UniProt ID: (Human) P01133

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Entrez Gene ID: (Human) 1950

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PeproGMP® Human EGF Recombinant Protein, PeproTech®

PeproGMP® Human EGF Protein (GMP100-15-100UG) in Functional

产品信息

GMP100-15-100UG

功能检测 (Functional)

体外检测 (IV)

其他PubMed (Misc)

种属

已发表种属

Expression System

氨基酸序列

分子量

分类

类型

纯度

内毒素浓度

活性

偶联物

形式

纯化类型

内含物

保存条件

运输条件

产品详细信息

靶标信息

生物信息学

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