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Wnt Surrogate-Fc Fusion Protein (PHG0401) in Functional
The Wnt surrogate transcriptome is highly similar to Wnt3a-regulated transcriptomes. Untreated iPSCs were cultured in DMEM/F-12 in the absence of Wnt agonist; StemFlex Medium is the PSC culture medium. All conditions were assayed 3 times as indicated by the labels on the left of the heat map. The heat map contains 4,744 genes with log2(rpm+1) and > 2.5-fold change in gene expression threshold; rpm = reads p... View More
产品信息
PHG0401
应用
建议稀释比
已发表文章
产品规格
种属
Human
已发表种属
Human
Expression System
HEK293 cells
分类
Recombinant
类型
Protein
纯度
> 95%
内毒素浓度
1 EU/mL
活性
Greater than 25 fold induction in Topflash assay activity at a Wnt surrogate-Fc protein concentration between 0.03 - 5.0 nM (based on dimeric stoichiometry)
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
purified
保存液
25mM tris, pH 8.2, with 500mM NaCl
内含物
no preservative
保存条件
-20°C or -80°C if preferred
运输条件
Dry ice
产品详细信息
Gibco Wnt Surrogate-Fc Fusion Protein activates similar Wnt signaling pathways as most commercially available recombinant Wnt3a proteins or Wnt-conditioned media, while requiring a fraction of the amount of protein or hassle. Gibco Wnt Surrogate-Fc Fusion Protein was engineered with improved solubility, overcoming a major obstacle of working with Wnt3a and reducing the concentrations required to activate Wnt signaling in your model system.
Gibco Wnt Surrogate-Fc Fusion Protein is comprised of the LRP-binding domain of DKK linked to a selective, high affinity binder of Frizzled receptors.
Gibco Wnt Surrogate-Fc Fusion Protein is provided at a concentration of 2 mg/mL which is equivalent to 17.1 µM based on dimeric stoichiometry.
Storage: Store undiluted Gibco Wnt Surrogate-Fc Fusion Protein at -80° for up to 1 year. After thawing, apportion into working aliquots and store at -20°C to -80°C for up to 2 months.
靶标信息
Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC119418; MGC119419; MGC119420; Protein Wnt-3a; wingless-type MMTV integration site family member 3A; wingless-type MMTV integration site family, member 3A; Wnt 3a; WNT3A protein
基因别名: WNT3A
UniProt ID: (Human) P56704
Entrez Gene ID: (Human) 89780
