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产品规格
检测灵敏度
3.2 pg/mL
测定范围
7.8-500
检测样本类型/体积
{Serum=20 µL, Supernatant=20 µL, Plasma=20 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
IFN alpha
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
Kit Overview
The Human IFN alpha ELISA kit enables the quantification of Human IFN alpha in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Human IFN alpha.
Principle of the assay
The Human IFN alpha solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : IFL, IFN, IFN-ALPHA, IFN-alphaA, IFN-alphaD, IFNA, IFNA1, IFNA13, IFNA2, IFNA2A, IFNA2B, IFNA2C, IFNA@, INFA2
基因ID : (Human) 3439, (Human) 3440
基因符号 : IFNA1, IFNA2
蛋白别名 : alpha-2a interferon, IFN-alpha 1b, IFN-alpha-1/13, IFN-alpha-2, interferon alpha 1b, interferon alpha 2a, interferon alpha 2b, interferon alpha A, Interferon alpha-1/13, Interferon alpha-2, Interferon alpha-A, Interferon alpha-D, interferon, alpha 1, alpha 2, interferon-alpha1, LeIF A, LeIF D, MGC138207, MGC138505, MGC138507, IFNα, Interferon alpha 13 (IFNA13), Interferon alpha leukocyte (IFL), Interferon alpha1 (IFNA1), Interferon alpha-1/13 (IFN-alpha-1/13), Interferon alpha-D (IFN alphaD), ifa, IFN alpha 1/13, IFN alpha 1b, IFN α, IFNAalphaIFN-alpha-1/13, IFNalpha
UniProt ID (Human) P01562, (Human) P01563
