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产品规格
检测灵敏度
<0.6 pg/mL
测定范围
4.69-300
检测样本类型/体积
{Serum=50 µL, Cell Lysate=50 µL, Plasma=50 µL, Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Biotinylated Detection antibody
Sample Diluent
Assay Buffer concentrate
Wash Buffer concentrate
SAV-HRP
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Biotinylated Detection antibody
Sample Diluent
Assay Buffer concentrate
Wash Buffer concentrate
SAV-HRP
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
PD-L1 (CD274)
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Kit Overview
The Human PD-L1 ELISA kit enables the quantification of Human PD-L1 in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Human PD-L1.
Principle of the assay
The Human PD-L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : B7-H, B7H1, CD274, PD-L1, PDCD1L1, PDCD1LG1, PDL1
基因ID : (Human) 29126
基因符号 : CD274
蛋白别名 : B7 homolog 1, B7-H1, CD274, CD274 antigen, PD-L1, PDCD1 ligand 1, Programmed cell death 1 ligand 1, sPD L1, pdl-1, sCD274, soluble CD274, soluble PD L1
UniProt ID (Human) Q9NZQ7
