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产品规格
检测灵敏度
20.81 pg/mL
测定范围
78.1-5000 pg/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL, Cell Lysate=50 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
8.5%
批内差异
6.6%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard (2 vials)
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
Standard (2 vials)
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Arginase 1
Target sub-specificity
Soluble
蛋白亚型
Adaptor Proteins
蛋白家族
Metabolic enzyme
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Arginase-1 Human ELISA Kit quantitates Hu Renin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Arginase-1.
原理方法
The Human Arginase-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : ARG1
基因ID : (Human) 383
基因符号 : ARG1
蛋白别名 : arginase, liver, liver-type arginase, type I arginase, A-I, Arginase, arginase 1 liver, Arginase1, HGNC:663, Liver Arginase, Type 1 Arginase, Arginase-1, Liver-type arginase, Type I arginase
UniProt ID (Human) P05089
