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产品规格
检测灵敏度
2 ng/mL
测定范围
2.05-500 ng/mL
检测样本类型/体积
{Plasma=33 µL, Serum=33 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
alpha-1 Antitrypsin
蛋白家族
Proteases Peptidases & Inhibitors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Serpin A1 ELISA quantitates Hu Serpin A1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Serpin A1.
原理方法
The Human Serpin A1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : A1A, A1AT, AAT, alpha1AT, PI, PI1, PRO0684, PRO2209, PRO2275, SERPINA1
基因ID : (Human) 5265
基因符号 : SERPINA1
蛋白别名 : alpha-1 antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1, SPAAT, a-1 AT, a1-antitrypsin, alpha 1 antitrypsin, Digestive Zymogen, MGC23330, MGC9222, Protease Serine 1, Serine Protease 1, TRP1, TRY1, Alpha-1-antitrypsin, alpha-1-antitrypsin null, protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin, serine (or cysteine) proteinase inhibitor, clade A, member 1, serpin peptidase inhibitor clade A member 1, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
UniProt ID (Human) P01009
