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Multispecies AKT/ERK/p70 S6K Activation InstantOne™ ELISA Kit
Representative data for ERK/AKT/p70 S6K Activation InstantOne™ ELISA. Left: A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor), washed and unstimulated (-) or stimulated with 10 ng/mL EGF for 10 min (+). Cells were analyzed for phosphorylated ERK 1/2/3 by InstantOne™ ELISA. Middle and Right: MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated w... View More
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产品规格
检测样本类型/体积
{Cell Lysate=20 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
运输条件
Wet ice
储存
2-8°C
蛋白名称
AKT Pan/Erk1/2/p70 S6 Kinase
均相（无需洗涤）
Human
检测试剂盒格式
InstantOne ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), AKT 1/2/3 (human, mouse, hamster), and p70 S6K (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
靶标信息
Extracellular signal-regulated kinase (ERK) is the founding member and a key component of the classical Mitogen-Activated Protein Kinase (MAPK) pathway. MEK activates ERK 1/2 by phosphorylation of its TxY motifs in response to a variety of stimuli, including growth factors and cytokines. AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. p70 S6 Kinase (p70S6K) is a member of the ribosomal S6 kinase family of Ser/Thr kinases. p70 S6K activity is controlled by multiple signaling pathways, including the MAPK, phosphoinositide-3 kinase (PI3K), and mTOR pathways. This regulation results in the phosphorylation of multiple sites of p70 S6K, including Thr389 within the linker domain, which is required for full activation. Activated p70 S6K phosphorylates several residues on the S6 ribosomal protein, thereby leading to increased protein synthesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : AKT, AKT1, AKT2, AKT3, CWS6, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, HIHGHH, HS44KDAP, HUMKER1A, MAPK1, MAPK2, MAPK3, MPPH, MPPH2, p38, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, p70 S6KA, p70(S6K)-alpha, p70-alpha, p70-S6K, PKB, PKB-ALPHA, PKB-GAMMA, PKBB, PKBBETA, PKBG, PRKBA, PRKBB, PRKBG, PRKM1, PRKM2, PRKM3, PS6K, RAC, RAC-ALPHA, RAC-BETA, RAC-gamma, RAC-PK-gamma, RPS6KB1, S6K, S6K-beta-1, S6K1, STK-2, STK14A
基因符号 : AKT1, AKT2, AKT3, MAPK1, MAPK3, RPS6KB1
蛋白别名 : 70 kDa ribosomal protein S6 kinase 1, AKT1m, ERK, pan AKT, s6k, ERK-1, ERK-2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, murine thymoma viral (v-akt) homolog-2, p42-MAPK, p44-ERK1, p44-MAPK, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6 kinase, alpha, P70S6K1, PKB, PKB alpha, PKB beta, PKB gamma, Protein kinase Akt-2, Protein kinase Akt-3, Protein kinase B, Protein kinase B alpha, Protein kinase B beta, Protein kinase B gamma, protein tyrosine kinase ERK2, Proto-oncogene c-Akt, putative v-akt murine thymoma viral oncoprotein 2, rac protein kinase alpha, RAC protein kinase beta, RAC-alpha serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-gamma serine/threonine protein kinase, RAC-gamma serine/threonine-protein kinase, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, ribosomal protein S6 kinase, 70kDa, polypeptide 1, S6K-beta-1, serine-threonine protein kinase, serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A, STK-2, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene homolog 2, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), v-akt murine thymoma viral oncogene-like protein 1
UniProt ID (Human) P31749, (Human) P31751, (Human) Q9Y243, (Human) P28482, (Human) P27361, (Human) P23443
