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LightShift™ EMSA 优化和控制试剂盒
The Thermo Scientific LightShift EMSA Optimization and Control Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility 了解更多信息
货号 20148X
价格（CNY)/ 100 reactions
价格2,042.00
飞享价1,735.90仅限网单直购,无需促销代码
(ends 31-Dec-2024)
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价格（CNY)/ 100 reactions
飞享价1,735.90仅限网单直购,无需促销代码
(ends 31-Dec-2024)
LightShift™ EMSA 优化和控制试剂盒
货号20148X
价格（CNY)/ 100 reactions
飞享价1,735.90仅限网单直购,无需促销代码
(ends 31-Dec-2024)
-
The Thermo Scientific LightShift EMSA Optimization and Control Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions. The kit includes reagents for setting up and customizing DNA binding reactions and a control set of DNA and protein extract to test the kit system.
Features of theLightShift EMSA Optimization and Control Kit:
• Excellent for detecting low-abundance proteins in nuclear extracts
• Sensitivity that surpasses radioactive and digoxigenin methods
• Compatible with previously established binding conditions for popular DNA-protein interactions
• Includes EBNA control system to help new users develop a working assay and understand the methods used to confirm binding interaction specificity
The principle for LightShift EMSA Detection is similar to a Western blot. Biotin end-labeled duplex DNA is incubated with a nuclear extract or purified factor and electrophoresed on a native gel. The DNA is then rapidly (30 minutes) transferred to a positive nylon membrane, UV crosslinked, probed with streptavidin-HRP conjugate and incubated with the substrate. The protocol from labeling to results can be accomplished in a single day.
The interaction of proteins with DNA is central to the control of many cellular processes including DNA replication, recombination and repair, transcription, and viral assembly. One technique that is central to studying gene regulation and determining protein:DNA interactions is the electrophoretic mobility shift assay (EMSA).
The EMSA technique is based on the observation that protein:DNA complexes migrate more slowly than free DNA molecules when subjected to non-denaturing polyacrylamide or agarose gel electrophoresis. Because the rate of DNA migration is shifted or retarded upon protein binding, the assay is also referred to as a gel shift or gel retardation assay. Until conception of the EMSA protein:DNA interactions were studied primarily by nitrocellulose filter-binding assays.
All that is needed to perform the assay is purified DNA target that has been end-labeled with biotin, the protein extract to be tested, nylon membrane and basic electrophoresis equipment. DNA targets can be synthesized with 5' or 3' biotin labels or they can be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Product No. 89818). Nuclear, cytosolic or whole cell protein extracts can be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Product No. 78833).
More Product Data
• Transfer EMSA gels using the Pierce G2 Fast Blotter
Related Products
LightShift™ Chemiluminescent EMSA Kit
LightShift™ Poly (dI-dC)
Features of theLightShift EMSA Optimization and Control Kit:
• Excellent for detecting low-abundance proteins in nuclear extracts
• Sensitivity that surpasses radioactive and digoxigenin methods
• Compatible with previously established binding conditions for popular DNA-protein interactions
• Includes EBNA control system to help new users develop a working assay and understand the methods used to confirm binding interaction specificity
The principle for LightShift EMSA Detection is similar to a Western blot. Biotin end-labeled duplex DNA is incubated with a nuclear extract or purified factor and electrophoresed on a native gel. The DNA is then rapidly (30 minutes) transferred to a positive nylon membrane, UV crosslinked, probed with streptavidin-HRP conjugate and incubated with the substrate. The protocol from labeling to results can be accomplished in a single day.
The interaction of proteins with DNA is central to the control of many cellular processes including DNA replication, recombination and repair, transcription, and viral assembly. One technique that is central to studying gene regulation and determining protein:DNA interactions is the electrophoretic mobility shift assay (EMSA).
The EMSA technique is based on the observation that protein:DNA complexes migrate more slowly than free DNA molecules when subjected to non-denaturing polyacrylamide or agarose gel electrophoresis. Because the rate of DNA migration is shifted or retarded upon protein binding, the assay is also referred to as a gel shift or gel retardation assay. Until conception of the EMSA protein:DNA interactions were studied primarily by nitrocellulose filter-binding assays.
All that is needed to perform the assay is purified DNA target that has been end-labeled with biotin, the protein extract to be tested, nylon membrane and basic electrophoresis equipment. DNA targets can be synthesized with 5' or 3' biotin labels or they can be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Product No. 89818). Nuclear, cytosolic or whole cell protein extracts can be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Product No. 78833).
More Product Data
• Transfer EMSA gels using the Pierce G2 Fast Blotter
Related Products
LightShift™ Chemiluminescent EMSA Kit
LightShift™ Poly (dI-dC)
仅供科研使用。不可用于诊断程序。
规格
检测EMSA Assay
产品规格试剂盒
包括10X 结合缓冲液 (1 mL)、生物素-EBNA 对照 DNA (50 µL)、未标记 EBNA DNA (50 µL)、EBNA 提取物 (125 µL)、Poly dI·dC (125 µL)、50% 甘油 (500 µL)、1% NP-40 (500 µL)、1 M KCl (1 mL)、100 mM MgCl2 (500 µL)、200 mM EDTA（pH 值 8.0；500 µL）、5X 上样缓冲液 (1 mL)
适用于（设备）myECL™ 成像仪、X 射线胶片
产品线LightShift™
产品类型LightShift EMSA 优化和控制试剂盒
数量100 次反应
靶标特异性无靶标特异性
技术凝胶迁移
Unit Size100 reactions
内容与储存
足够用于：100 次结合反应
• 10X 结合缓冲液，1 mL
•生物素-EBNA 控制 DNA，50 µL
•未标记 EBNA DNA，50 µL
• EBNA 提取物，125 µL
• Poly (dI·dC)，125 µL
• 50% 甘油，500 µL
• 1% NP-40，500 µL
• 1 M KCl，1 mL
• 100 mM MgCl2，500 µL
• 200 mM EDTA pH 值 8.0，500 µL
• 5X 上样缓冲液，1 mL
在 -20°C 下储存。
• 10X 结合缓冲液，1 mL
•生物素-EBNA 控制 DNA，50 µL
•未标记 EBNA DNA，50 µL
• EBNA 提取物，125 µL
• Poly (dI·dC)，125 µL
• 50% 甘油，500 µL
• 1% NP-40，500 µL
• 1 M KCl，1 mL
• 100 mM MgCl2，500 µL
• 200 mM EDTA pH 值 8.0，500 µL
• 5X 上样缓冲液，1 mL
在 -20°C 下储存。