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Invitrogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
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Rabbit IgG (H+L) Secondary Antibody (31460) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1) and Hep G2 (Lane 2). The blots were probed with Anti-CacyBP Rabbit Polyclonal Antibody (Product # 720326, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # 31460) at dilutions 1:10,000 (Fig. 1), 1:100,000 (Fig. 2) and 1:200,000 (Fig. 3). A 30 kDa b... View More
产品信息
31460
应用
建议稀释比
免疫印迹 (WB)
1:10,000-1:200,000
免疫组化（石蜡） (IHC (P))
Assay-dependent
酶联免疫吸附实验 (ELISA)
1:5,000-1:10,000
免疫沉淀 (IP)
1:500-1:5,000
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Purified Rabbit IgG, whole molecule
偶联物
形式
Lyophilized
浓度
0.8 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6, with 15mg/mL BSA, 50mM sucrose
内含物
no preservative
保存条件
4° C
运输条件
Ambient
RRID
AB_228341
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Product # 31460 has been successfully used in Western blot, IHC and IP applications.
Product # 31460 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species and with SuperBlock® Blocking Buffers.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 2.0 mL of distilled water (0.8 mg/mL after restoration).
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
