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Invitrogen
Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet™ 786, eBioscience™
Application
图: 1 / 1
Rat IgG1 kappa Isotype Control (417-4301-81) in Flow
BALB/c mouse splenocytes were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0041-82) and staining buffer (autofluorescence) (left) or 0.5 µg of Rat IgG1 kappa Isotype Control, Brilliant Violet 786 (Product # 417-4301-81) (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
产品信息
417-4301-81
应用
建议稀释比
流式细胞分析 (Flow)
Assay-dependent
对照试剂 (Ctrl)
Assay-dependent
产品规格
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eBRG1
激发/发射光谱
406/788 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description
The monoclonal rat IgG1 kappa is useful as an isotype control immunoglobulin.
Applications Tested
This eBRG1 antibody has been tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes. Use at the same concentration as the experimental antibody.
Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity
This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Our internal testing suggests that Brilliant Violet™ 786 (BV786) is not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 786 nm; Laser: Violet Laser.
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
靶标信息
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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