Thermo Scientific™

Melon™ 凝胶 IgG 纯化试剂盒

货号: 45212
Thermo Scientific™

Melon™ 凝胶 IgG 纯化试剂盒

货号: 45212
货号
45212
单位规格
25 mL
价格(CNY)
价格 11,135.00
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库存状况
-
数量
货号单位规格价格(CNY)库存状况数量
4521225 mL
价格 11,135.00
您的价格
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The Thermo Scientific Melon Gel IgG Purification Kit offers a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Features of Melon Gel IgG Purification Kits:

Simple, one-step protocol—IgG purification with Melon Gel Resin does not involve binding, washing, and multiple elution steps required by Protein A and Protein G
Rapid purification—antibody purification from serum is four to six times faster than Protein A or G methods
High recovery and purity—for many species of serum antibody recovery is greater than 90% with purity greater than 80%
Minimal dilution—reduced need to concentrate antibody before storage or use
Robust purification—Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G
Gentle purification—No harsh antibody elution conditions helps retain IgG activity
Regenerable support—Melon Gel Resin can be used for multiple antibody purifications

Melon Gel IgG Purification Kits enable purification of antibodies from serum and other sample types. Melon Gel Resin binds and removes serum proteins, thereby allowing pure IgG to be collected in the flow-through fraction. The procedure is gentle because no harsh elution conditions are involved.

Melon Gel Resin contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because Melon Gel Resin is not a bind-and-release support, it is extremely fast and gentle to ant ibodies, resulting in antibody preparations of high purity and high activity.

Melon Gel Resin works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with Melon Gel Resin are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.

Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Related Products
Melon™ Gel IgG Spin Purification Kit
Melon™ Gel Purification Buffer
Melon™ Gel Regenerant
For Research Use Only. Not for use in diagnostic procedures.

规格

产品类型
IgG 纯化试剂盒
产品线
Melon™
色谱柱类型
具有专利技术的树脂、亲和性
产品规格
试剂盒、瓶
固定相
具有专利技术的配体
纯化目标
IgG 抗体
描述
Melon 凝胶 IgG 纯化试剂盒
数量
25 mL

内容与储存

足够用于:从 75 到 150 mL 血清中纯化 IgG(总计从血清中纯化约 2 g IgG)
• Melon 凝胶支持物,25 mL
• Melon 凝胶纯化缓冲液(干燥混合物,可制备 100X 制剂),1 L
• Melon 凝胶再生剂(干燥混合物,可制备 1X 制剂),1 L

储存在 4 C°下

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