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Citations & References (18)
Thermo Scientific™
EquiPhi29™ DNA Polymerase
Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution. This enzymeRead more
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| Catalog Number | Quantity | ProductName |
|---|---|---|
| A39391 | 1000 U | |
| A39390 | 250 U | |
| A39392 | 5000 U |
Catalog number A39391
Price (CNY)
2,167.00
Online Exclusive
Ends: 31-Dec-2025
3,095.00Save 928.00 (30%)
Each
Quantity:
1000 U
Price (CNY)
2,167.00
Online Exclusive
Ends: 31-Dec-2025
3,095.00Save 928.00 (30%)
Each
Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution. This enzyme is significantly improved over phi29 DNA polymerase in protein thermostability, reaction speed, product yield, and amplification bias, while retaining all the benefits of the wild-type enzyme.
EquiPhi29 DNA Polymerase possesses strong strand displacement activity and 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.
Features of EquiPhi29 DNA Polymerase
• Highest processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized
• Low amplification bias
• Extremely high yields of amplified DNA, even from small amounts of template
• Highly accurate DNA synthesis in a short time
Applications
EquiPhi29 DNA Polymerase may be used in a wide variety of applications including:
• Unbiased whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Protein-primed DNA amplification
• Cell-free cloning of lethal DNA
• In situ genotyping with padlock probes
• RNA-primed DNA amplification
Notes
1. The addition of pyrophosphatase to the reaction mixture with EquiPhi29 DNA Polymerase may further enhance DNA synthesis
2. Because of the enzyme's 3'→5' proofreading exonuclease, 3’-modified primers are highly recommended.
3. EquiPhi29 DNA Polymerase can amplify DNA in a wide range of temperatures (30–45°C), with an optimal reaction temperature of 42°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10 U/μL
Optimal Reaction Temperature42°C
Reaction Time2 hours
Shipping ConditionDry Ice
For Use With (Application)MDA-WGA, RCA
PolymeraseEquiPhi29 DNA Polymerase
Product TypeStand-alone enzyme
Quantity1000 U
Unit SizeEach
Contents & Storage
• EquiPhi29 DNA Polymerase (100 μL at 10 U/μL)
• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1.0 mL)
• 110 mM DTT (0.25 mL)
Store at –15 to –25°C.
Frequently asked questions (FAQs)
What is the error rate of EquiPhi29 DNA Polymerase?
Can I use EquiPhi29 DNA Polymerase to incorporate 5-methyl-dCTP?
When using EquiPhi29 DNA Polymerase, do I need to purify amplified DNA products before downstream applications?
What is the minimal recommended time for amplification with EquiPhi29 DNA Polymerase?
Can liquid media culture or colonies be used as a starting material for amplification with EquiPhi29 DNA Polymerase?
Citations & References (18)
Citations & References
Abstract
Fidelity of phi 29 DNA polymerase. Comparison between protein-primed initiation and DNA polymerization.
Journal:J Biol Chem
PubMed ID:8428945
'Phi 29 DNA polymerase is able to catalyze two different synthetic reactions: protein-primed initiation and DNA polymerization. We have studied the fidelity of phi 29 DNA polymerase when carrying out these two reactions. Global fidelity was dissected into three steps: insertion discrimination, mismatch elongation, and proofreading. The insertion discrimination of
Whole-metagenome amplification of a microbial community associated with scleractinian coral by multiple displacement amplification using phi29 polymerase.
Journal:Environ Microbiol
PubMed ID:16817924
'Limitations in obtaining sufficient specimens and difficulties in extracting high quality DNA from environmental samples have impeded understanding of the structure of microbial communities. In this study, multiple displacement amplification (MDA) using phi29 polymerase was applied to overcome these hindrances. Optimization of the reaction conditions for amplification of the bacterial
Suitability of genomic DNA synthesized by strand displacement amplification (SDA) for AFLP analysis: genotyping single spores of arbuscular mycorrhizal (AM) fungi.
Journal:J Microbiol Methods
PubMed ID:15936100
'Limited biological samples of microbial origin often yield insufficient amounts of genomic DNA, making application of standard techniques of genetic analysis, like amplified fragment length polymorphism (AFLP), virtually impossible. The Phi29 DNA polymerase based whole genome amplification (WGA) method has the potential to alleviate this technical bottleneck. In the present
Novel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCA.
Journal:RNA
PubMed ID:19244362
We present a novel Phi29 DNA polymerase application in RCA-based target RNA detection and analysis. The 3'-->5' RNase activity of Phi29 DNA polymerase converts target RNA into a primer and the polymerase uses this newly generated primer for RCA initiation. Therefore, using target RNA-primed RCA, padlock probes may be targeted
Towards the analysis of the genomes of single cells: further characterisation of the multiple displacement amplification.
Journal:Gene
PubMed ID:16564650
The development of methods for the analysis and comparison of the nucleic acids contained in single cells is an ambitious and challenging goal that may provide useful insights in many physiopathological processes. We review here some of the published protocols for the amplification of whole genomes (WGA). We focus on