This substrate bundle contains SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate
Thermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.

• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

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SuperSignal West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis. It is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for routine western blotting applications.

• Sensitivity: picogram to femtogram
• Stability: eight hours for working solution; one year for kit at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

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