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Invitrogen
Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 647
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A48272) in ICC/IF
Immunofluorescent analysis of tubulin in HeLa cells. The cells were fixed with 4% formaldehyde for 20 mins, washed 3x in PBS, permeabilized with 0.5% Triton X-100 in PBS for 20 minutes, washed 3x in PBS and blocked with 3% BSA in PBS for 30 minutes. Cells were incubated with Rat anti-tubulin (1:1000) and and mouse anti-ATPIF1 (1:250) (Product # A-21355) in 3% BSA in PBS overnight at 4C and washed 3x in PBS.... View More
产品信息
A48272
应用
建议稀释比
免疫印迹 (WB)
1:10,000
免疫细胞化学 (ICC/IF)
1-10 µg/mL
产品规格
种属反应
Rat
宿主/亚型
Donkey
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
激发/发射光谱
658/675 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.5
内含物
0.016% Methylisothiazolone, 0.016% Bromonitrodioxane
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2893138
靶标
IgG
交叉吸附
against serum proteins from bovine, goat, rabbit, mouse, chicken, guinea pig, hamster, horse, sheep, and human.
抗体形式
Whole Antibody
产品详细信息
To minimize cross-reactivity, the donkey anti-rat IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, goat, rabbit, mouse, chicken, guinea pig, hamster, horse, sheep, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.
Specificity: This antibody binds to heavy chains on rat IgG and light chains on all rat immunoglobulins. This antibody does not bind non-immunoglobulin rat serum proteins or IgG from bovine, goat, rabbit, mouse, chicken, guinea pig, hamster, horse, sheep, or human.
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
